Multifunctional Poly(2-oxazoline) Nanoparticles for Biological Applications (pages 1869–1873)
Kristian Kempe, Antje Vollrath, Hendrik W. Schaefer, Tobias G. Poehlmann, Christoph Biskup, Richard Hoogenboom, Stephanie Hornig and Ulrich S. Schubert
Article first published online: 25 OCT 2010 | DOI: 10.1002/marc.201000283
Multifunctional copoly(2-oxazoline) nanoparticles were prepared that were taken up by cells as revealed by confocal laser scanning microscopy measurements. The polymer contains double bond side chains as potential reaction sites for “thio”-click reactions as well as a fluorescein label covalently bound to the polymer backbone. Using the nanoprecipitation technique, spherical nanoparticles of 200–800 nm were obtained. Confocal laser scanning microscopy measurements and flow cytometry shows uptake of the particles into the cells without any toxic effect.