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Characterization of intact microorganisms by MALDI mass spectrometry

Authors

  • Catherine Fenselau,

    Corresponding author
    1. Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742
    • Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742.
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  • Plamen A. Demirev

    Corresponding author
    1. Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742
    • Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742.
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Abstract

I.Introduction157
II.MALDI-TOF Spectra of Biomarkers158
III.Instrumentation158
IV.Sample Introduction160
V.Preparation of Microorganism Samples for MALDI Analysis161
VI.Characterization of MALDI Biomarkers162
VII.Data Analysis: Fingerprint Libraries, Statistical Methods, Bioinformatics163
VIII.Applications165
IX.Summary and Envoi166
Acknowledgments166
References166

The application of MALDI mass spectrometry to desorb protein biomarkers from intact viruses, bacteria, fungus, and spores is the focus of this review. Instrumentation, sample collection, sample preparation, and algorithms for data analysis are summarized. Optimally these analyses should be carried out in less than five minutes. Successful applications are discussed from biotechnology, cell biology, and the pharmaceutical industry. © 2002 John Wiley & Sons, Inc., Mass Spec Rev 20:157–171, 2001

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