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Peptide mapping of proteins in human body fluids using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Authors

  • Jonas Bergquist,

    Corresponding author
    1. Department of Analytical Chemistry, Institute of Chemistry, Uppsala University, P.O. Box 531, SE-751 21 Uppsala, Sweden
    2. Department of Psychiatry and Neurochemistry, Institute of Clinical Neuroscience, Göteborg University, Sahlgrenska University Hospital, Mölndal, Sweden
    • Department of Analytical Chemistry, Institute of Chemistry, Uppsala University, P.O. Box 531, SE-751 21 Uppsala, Sweden.
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  • Magnus Palmblad,

    1. The Ångström Laboratory, Division of Ion Physics, Uppsala University, Uppsala, Sweden
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  • Magnus Wetterhall,

    1. Department of Analytical Chemistry, Institute of Chemistry, Uppsala University, P.O. Box 531, SE-751 21 Uppsala, Sweden
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  • Per Håkansson,

    1. The Ångström Laboratory, Division of Ion Physics, Uppsala University, Uppsala, Sweden
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  • Karin E. Markides

    1. Department of Analytical Chemistry, Institute of Chemistry, Uppsala University, P.O. Box 531, SE-751 21 Uppsala, Sweden
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Abstract

1

Table 1. 
 I.Introduction3
 A. Human Body Fluids as Sources for Clinical Markers3
 B. Plasma3
 C. Cerebrospinal Fluid5
 D. Saliva5
 E. Urine5
 II.Review of Typical Materials and Methods5
 A. General5
 B. Human Plasma Samples5
 C. Human CSF Samples5
 D. Human Saliva Samples6
 E. Human Urine Samples6
 F. Tryptic Digestion of Proteins6
 G. Desalting6
 H. Direct Infusion and Capillary Interface6
 I. Mass Spectrometry6
 J. Data Analysis7
III.Results and Discussion7
IV.Conclusions12
Acknowledgments13
References13

Human body fluids have been rediscovered in the post-genomic era as great sources of biological markers and perhaps particularly as sources of potential protein biomarkers of disease. Analytical tools that allow rapid screening, low sample consumption, and accurate protein identification are of great importance in studies of complex biological samples and clinical diagnosis. Mass spectrometry is today one of the most important analytical tools with applications in a wide variety of fields. One of the fastest growing applications is in proteomics, or the study of protein expression in an organism. Mass spectrometry has been used to find post-translational modifications and to identify key functions of proteins in the human body. In this study, we review the use of human body fluids as sources for clinical markers and present new data that show the ability of Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) to identify and characterize proteins in four human body fluids: plasma, cerebrospinal fluid (CSF), saliva, and urine. The body fluids were tryptically digested without any prior separation, purification, or selection, and the digest was introduced into a 9.4 T FTICR mass spectrometer by direct-infusion electrospray ionization (ESI). Even though these samples represent complex biological mixtures, the described method provides information that is comparable with traditional 2D-PAGE data. The sample consumption is extremely low, a few microliters, and the analysis time is only a few minutes. It is, however, evident that the separation of proteins and/or peptides must be included in the methodology, in order to detect low-abundance proteins and other proteins of biological relevance. © 2002 Wiley Periodicals, Inc. Mass Spec Rev 21:2–15, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mas.10016

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