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Keywords:

  • SELDI;
  • SEND;
  • ProteinChip;
  • surface enhanced laser desorption ionization

Abstract

    I.Introduction34
   II.Affinity Probes35
 A.  Initial Surface-Affinity Research36
 B.  Self-Assembled Monolayers36
 C.  Dextran36
 D.  Metal Affinity36
 E.  Antibody- and Protein-Affinity Probes37
 F.  Lectin Affinity Capture38
 G.  Inherent Hydrophobic Interaction Bio-Probes38
 H.  Bio-Molecular Interaction Probes39
 III.SELDI39
 A.  Fundamentals of SELDI39
 B.  SELDI in Proteomics40
 IV.Conclusions41
References41

The overall history and recent advancements in Surface-Enhanced Laser Desorption/Ionization (SELDI) affinity technology is reviewed. A detailed account of SELDI technology, utilizing Immobilized-Metal Affinity surfaces, pseudo-specific chromatographic surfaces, and biospecific interactive surfaces, is presented with particular emphasis placed upon examination of fundamental characteristics as well as specific applications for each. Finally, a detailed review of the specific use of such affinity surfaces in fundamental aspects of clinical, process, and research proteomics activity is presented. © 2003 Wiley Periodicals, Inc., Mass Spec Rev 23:34–44, 2004