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Metabolomic applications of HILIC–LC–MS
Article first published online: 25 JUN 2009
DOI: 10.1002/mas.20252
Copyright © 2009 Wiley Periodicals, Inc.
1098-2787/asset/cover.gif?v=1&s=11bb10c63a9aa71025a291f9f99aaede7642bd95 "Mass Spectrometry Reviews")
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How to Cite
Cubbon, S., Antonio, C., Wilson, J. and Thomas-Oates, J. (2010), Metabolomic applications of HILIC–LC–MS. Mass Spectrom. Rev., 29: 671–684. doi: 10.1002/mas.20252
Publication History
- Issue published online: 17 AUG 2010
- Article first published online: 25 JUN 2009
- Manuscript Revised: 30 MAR 2009
- Manuscript Accepted: 30 MAR 2009
- Manuscript Received: 29 NOV 2008
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Keywords:
- hydrophilic interaction liquid chromatography (HILIC);
- LC–MS;
- metabolomics;
- metabonomics;
- plant metabolites;
- urine;
- principle component analysis (PCA);
- multivariate analysis (MVA)
Abstract
Hydrophilic interaction liquid chromatography (HILIC), although not a new technique, has enjoyed a recent renaissance with the introduction of robust and reproducible stationary phases. It is consequently finding application in metabolomics studies, which have traditionally relied on the stability of reversed phases (RPs), since the biofluids analyzed are predominantly aqueous and thus contain many polar analytes. HILIC's retention of those polar compounds and use of solvents readily compatible with mass spectrometry have seen its increasing adoption in studies of complex aqueous metabolomes. This review describes the stationary phases and their features, surveys HILIC–LC–MS's role in metabolomics experiments, discusses approaches to data extraction and analysis including multivariate analysis, and reviews the literature on HILIC–MS applications in metabolomics. © 2009 Wiley Periodicals, Inc., Mass Spec Rev 29:671–684, 2010