Multi-dimensional mass spectrometry-based shotgun lipidomics and novel strategies for lipidomic analyses

Authors

  • Xianlin Han,

    Corresponding author
    1. Sanford-Burnham Medical Research Institute, Orlando, FL 32827
    • Sanford-Burnham Medical Research Institute, 6400 Sanger Road, Orlando, FL 32827.
    Search for more papers by this author
  • Kui Yang,

    1. Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110
    Search for more papers by this author
  • Richard W. Gross

    1. Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110
    2. Department of Chemistry, Washington University, St. Louis, MO 63130
    Search for more papers by this author

Abstract

Since our last comprehensive review on multi-dimensional mass spectrometry-based shotgun lipidomics (Mass Spectrom. Rev. 24 (2005), 367), many new developments in the field of lipidomics have occurred. These developments include new strategies and refinements for shotgun lipidomic approaches that use direct infusion, including novel fragmentation strategies, identification of multiple new informative dimensions for mass spectrometric interrogation, and the development of new bioinformatic approaches for enhanced identification and quantitation of the individual molecular constituents that comprise each cell's lipidome. Concurrently, advances in liquid chromatography-based platforms and novel strategies for quantitative matrix-assisted laser desorption/ionization mass spectrometry for lipidomic analyses have been developed. Through the synergistic use of this repertoire of new mass spectrometric approaches, the power and scope of lipidomics has been greatly expanded to accelerate progress toward the comprehensive understanding of the pleiotropic roles of lipids in biological systems. © 2011 Wiley Periodicals, Inc., Mass Spec Rev 31:134–178, 2012

Ancillary