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Structural characterization of bacterial lipopolysaccharides with mass spectrometry and on- and off-line separation techniques

Authors

  • Anikó Kilár,

    Corresponding author
    1. Department of Analytical and Environmental Chemistry, Institute of Chemistry, Faculty of Sciences, University of Pécs, Ifjúság u. 6., H-7624 Pécs, Hungary
    • Department of Analytical and Environmental Chemistry, Institute of Chemistry, Faculty of Sciences, University of Pécs, Ifjúság u. 6., H-7624 Pécs, Hungary.
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  • Ágnes Dörnyei,

    Corresponding author
    1. Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary
    • Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary.
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  • Béla Kocsis

    1. Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary
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Abstract

The focus of this review is the application of mass spectrometry to the structural characterization of bacterial lipopolysaccharides (LPSs), also referred to as “endotoxins,” because they elicit the strong immune response in infected organisms. Recently, a wide variety of MS-based applications have been implemented to the structure elucidation of LPS. Methodological improvements, as well as on- and off-line separation procedures, proved the versatility of mass spectrometry to study complex LPS mixtures. Special attention is given in the review to the tandem mass spectrometric methods and protocols for the analyses of lipid A, the endotoxic principle of LPS. We compare and evaluate the different ionization techniques (MALDI, ESI) in view of their use in intact R- and S-type LPS and lipid A studies. Methods for sample preparation of LPS prior to mass spectrometric analysis are also described. The direct identification of intrinsic heterogeneities of most intact LPS and lipid A preparations is a particular challenge, for which separation techniques (e.g., TLC, slab-PAGE, CE, GC, HPLC) combined with mass spectrometry are often necessary. A brief summary of these combined methodologies to profile LPS molecular species is provided. © 2012 Wiley Periodicals, Inc., Mass Spec Rev 32:90–117, 2013

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