Graziano Colombo and Marco Clerici contributed equally to this work.
Pathophysiology of tobacco smoke exposure: Recent insights from comparative and redox proteomics
Article first published online: 22 NOV 2013
© 2013 Wiley Periodicals, Inc.
Mass Spectrometry Reviews
Special Issue: Special Issue on Redox Proteomics Part 3
Volume 33, Issue 3, pages 183–218, May/June 2014
How to Cite
Colombo, G., Clerici, M., Giustarini, D., Portinaro, N. M., Aldini, G., Rossi, R., Milzani, A. and Dalle-Donne, I. (2014), Pathophysiology of tobacco smoke exposure: Recent insights from comparative and redox proteomics. Mass Spectrom. Rev., 33: 183–218. doi: 10.1002/mas.21392
- Issue published online: 28 MAR 2014
- Article first published online: 22 NOV 2013
- Manuscript Revised: 23 MAY 2013
- Manuscript Accepted: 23 MAY 2013
- Manuscript Received: 27 APR 2013
- Fondazione Ariel
- tobacco smoke;
- (redox) proteomics;
- mass spectrometry;
- oxidative modifications;
- protein oxidation
First-hand and second-hand tobacco smoke are causally linked to a huge number of deaths and are responsible for a broad spectrum of pathologies such as cancer, cardiovascular, respiratory, and eye diseases as well as adverse effects on female reproductive function. Cigarette smoke is a complex mixture of thousands of different chemical species, which exert their negative effects on macromolecules and biochemical pathways, both directly and indirectly. Many compounds can act as oxidants, pro-inflammatory agents, carcinogens, or a combination of these. The redox behavior of cigarette smoke has many implications for smoke related diseases. Reactive oxygen and nitrogen species (both radicals and non-radicals), reactive carbonyl compounds, and other species may induce oxidative damage in almost all the biological macromolecules, compromising their structure and/or function. Different quantitative and redox proteomic approaches have been applied in vitro and in vivo to evaluate, respectively, changes in protein expression and specific oxidative protein modifications induced by exposure to cigarette smoke and are overviewed in this review. Many gel-based and gel-free proteomic techniques have already been used successfully to obtain clues about smoke effects on different proteins in cell cultures, animal models, and humans. The further implementation with other sensitive screening techniques could be useful to integrate the comprehension of cigarette smoke effects on human health. In particular, the redox proteomic approach may also help identify biomarkers of exposure to tobacco smoke useful for preventing these effects or potentially predictive of the onset and/or progression of smoking-induced diseases as well as potential targets for therapeutic strategies. © 2013 Wiley Periodicals, Inc. Mass Spec Rev 33: 183–218, 2014.