Temperature-responsive, polymer-modified surfaces for green chromatography

Authors

  • Akihiko Kikuchi,

    1. Institute of Advanced Biomedical Engineering & Science, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan
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  • Teruo Okano

    1. Institute of Advanced Biomedical Engineering & Science, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan
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Abstract

Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) and its derivatives were utilized as chromatography column matrix modifiers to develop novel supports for thermoresponsive hydrophobic chromatography with aqueous mobile phase. In the column, matrix surfaces show thermoresponsive hydrophilic/hydrophobic property alterations, which alter interaction with and retention of solutes to be separated. We have also demonstrated that the electrostatic interaction of ionic solutes and charged, thermoresponsive polymer-modified surfaces can be modulated temperature changes in the aqueous mobile phase alone.

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