Synthesis, Characterization of Copper-Loaded Carboxymethyl-Chitosan Nanoparticles with Effective Antibacterial Activity

Authors

  • Chunju Gu,

    1. State Key Lab for Modification of Chemical Fibers and Polymer Materials, College of Material Sci. & Eng., Donghua University, Shanghai 201620, China
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  • Bin Sun,

    1. State Key Lab for Modification of Chemical Fibers and Polymer Materials, College of Material Sci. & Eng., Donghua University, Shanghai 201620, China
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  • Wenhua Wu,

    1. State Key Lab for Modification of Chemical Fibers and Polymer Materials, College of Material Sci. & Eng., Donghua University, Shanghai 201620, China
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  • Fengchuan Wang,

    1. State Key Lab for Modification of Chemical Fibers and Polymer Materials, College of Material Sci. & Eng., Donghua University, Shanghai 201620, China
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  • Meifang Zhu

    Corresponding author
    1. State Key Lab for Modification of Chemical Fibers and Polymer Materials, College of Material Sci. & Eng., Donghua University, Shanghai 201620, China
    • State Key Lab for Modification of Chemical Fibers and Polymer Materials, College of Material Sci. & Eng., Donghua University, Shanghai 201620, China
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Abstract

Summary: Copper-loaded carboxymethyl-chitosan (CMCS-Cu) nanoparticles were successfully prepared by chelation under aqueous conditions. The effect of degree of deacetylation and substitution, the molecular weight of CMCS, CMCS concentration, Cu(II) ions concentration, pH value of the solution, as well as temperature, on the morphology of the yielded particles were systematically investigated. The physicochemical properties of the particles were determined by size and zeta potential analysis, FTIR analysis, DLS, TEM, SEM and XRD pattern. FTIR and XRD revealed that Cu (II) ions and CMCS formed a chelate complex. The size of CMCS-Cu particles shows a good consistency by DLS, TEM, and SEM. The nanoparticles with the size of about 70 nm have been prepared at 0.13 wt% CMCS, 16 mmol/L Cu(II) ions, pH value 4.56 at 25 °C. The antibacterial activity of CMCS, CMCS-Cu normal particles with the size of about 1000 nm and CMCS-Cu nanoparticles with the size of less than 100 nm against Staphylococcus aureus was evaluated by vibration method. Results show that the antibacterial efficiency of nanoparticles reached 99%, which is much more efficient than 68.9% of the normal one and 6.1% of CMCS. CMCS-Cu nanoparticles were proved to be a good novel antibacterial material.

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