mbo3101-sup-0001-FigureS1.jpgimage/jpg98KFigure S1. TolQ-dependent cell filamentation is not mitigated by concurrent expression of TolR; whereas the TolQ paralogue ExbB does not induce filamentation when overexpressed. Figure S1A: Stained preparations of W3110 cells bearing plasmids carrying either the l-arabinose-regulated plasmid pBAD24 (pBAD24) or pBAD24 derivatives encoding tolQ (ptolQ), tolQR (ptolR), or exbBD (pexbBD) genes under the control of the pBAD promoter grown for 24 h at 37°C with aeration in Miller LB supplemented with 100 μg mL−1 ampicillin and either no l-arabinose or 0.1% (w/v) l-arabinose are shown. All panels are displayed at the same relative magnification, with a bar representing 10 μm provided in each panel for scale. Figure S1B: Immunoblot analysis of samples from cells used for stained preparations in Figure S1A (above). Samples were prepared by TCA precipitation as described in 'Methods', then resolved by SDS-PAGE on 11% polyacrylamide gels, transferred to a PVDF membrane, and visualized by enhanced chemiluminescence using a monospecific anti-TolQ antiserum and a polyspecific anti-ExbB antiserum as described in 'Methods'. The positions of molecular mass standards are indicated as kDa values between the developed blot. Two nonspecific bands are evident in the samples probed with anti-ExbB (indicated by “*”), with the ExbB band evident at 25 kDa.

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