Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome
Version of Record online: 25 JUL 2013
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 5, pages 717–724, October 2013
How to Cite
MicrobiologyOpen 2013; 2(5): 717–724
- Issue online: 8 OCT 2013
- Version of Record online: 25 JUL 2013
- Manuscript Accepted: 17 JUN 2013
- Manuscript Revised: 12 JUN 2013
- Manuscript Received: 15 APR 2013
- Department of Agriculture and Food
- SFI TIDA
- Glucose dehydrogenase;
- MPS ;
- pyrroloquinoline quinone;
- soil metagenome.
Mineral phosphate solubilization (MPS) microorganisms are important for their provision of orthophosphate anions for plant growth promotion activity in soil. In this study, we applied a functional metagenomic approach to identify this trait directly from the microbiome in barley rhizosphere soil that had not received P fertilizer over a 15-year period. A fosmid system was used to clone the metagenome of which 18,000 clones (~666 Mb of DNA) was screened for MPS. Functional assays and High Performance Liquid Chromatography analysis recognized gluconic acid production and MPS activity in the range 24.8–77.1 mmol/L and 27.6–38.16 μg/mL, respectively, when screened in an Escherichia coli host (at frequency of one MPS-positive clone hit per 114 Mb DNA tested). The MPS clones (with average insert size of ~37 kb) were analysed by 454 Roche sequencing and annotated. A number of genes/operons with homology to Phosphorous (P) uptake, regulatory and solubilization mechanisms were identified, linking the MPS function to the uncultivated microbiome present in barley rhizosphere soil.