mbo3113-sup-0001-FigS1.tifimage/tif1250KFigure S1. The mtl2Δ cells are hypersensitive to different types of cell wall stress. Equal numbers of wild-type, wsc1Δ and mtl2Δ cells were diluted and (4 × 104, 2 × 104, 2 × 102 and 2 × 101 cells respectively) were spotted onto YES plates without and with caffeine (15 mmol/L), sodium orthovanadate (1.7 mmol/L), SDS (0.015%), hydrogen peroxide (0.8 mmol/L) and NaCl (100 mmol/L). Colony formation was analysed after 3 days of incubation at 28ºC.
mbo3113-sup-0002-FigS2.tifimage/tif10527KFigure S2. Localization of Wsc1p-GFP and Mtl2p-GFP to the cortex is not altered by microtubule depolymerisation. (A) Cells expressing Wsc1p-GFP and mCherry-atb2 (SC240) were treated with dimethyl sulfoxide (1%) (upper panels) or 25 μg/mL MBC (lower panels) for 20 min and then analysed for Wsc1p-GFP localization (left), mCherry-atb2 (middle) or Nomarski (right). (B) Cells expressing Mtl2p-GFP and mCherry-atb2 (SC242) were treated and analysed as above.
mbo3113-sup-0003-FigS3.tifimage/tif4367KFigure S3. The distribution of Rgf1p-GFP, Rgf2p-GFP and Rgf3p-GFP is not affected in strains deleted for wsc1+. (Upper panel) localization of Rgf1p-GFP in wild-type (PG40) and wsc1Δ cells (SC165). (Middle panel) localization of Rgf2p-GFP, in wild-type (HVP54 + p41X-Rgf2-GFP) and wsc1Δ cells (SC136+ p41X-Rgf2-GFP). (Bottom panel) localization of Rgf3p-GFP in wild-type (SC238) and wsc1Δ cells (SC198). Cells were grown to log phase in YES medium at 28ºC.

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