The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway
Version of Record online: 30 JUL 2013
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 5, pages 778–794, October 2013
How to Cite
MicrobiologyOpen 2013; 2(5): 778–794
- Issue online: 8 OCT 2013
- Version of Record online: 30 JUL 2013
- Manuscript Accepted: 11 JUN 2013
- Manuscript Revised: 7 JUN 2013
- Manuscript Received: 22 APR 2013
- CICYT. Grant Numbers: BFU2008-00963/BMC, BFU2011-24683/BMC
- Junta de Castilla y León. Grant Number: GR231
- Ramón Areces Foundation
|mbo3113-sup-0001-FigS1.tif||image/tif||1250K||Figure S1. The mtl2Δ cells are hypersensitive to different types of cell wall stress. Equal numbers of wild-type, wsc1Δ and mtl2Δ cells were diluted and (4 × 104, 2 × 104, 2 × 102 and 2 × 101 cells respectively) were spotted onto YES plates without and with caffeine (15 mmol/L), sodium orthovanadate (1.7 mmol/L), SDS (0.015%), hydrogen peroxide (0.8 mmol/L) and NaCl (100 mmol/L). Colony formation was analysed after 3 days of incubation at 28ºC.|
|mbo3113-sup-0002-FigS2.tif||image/tif||10527K||Figure S2. Localization of Wsc1p-GFP and Mtl2p-GFP to the cortex is not altered by microtubule depolymerisation. (A) Cells expressing Wsc1p-GFP and mCherry-atb2 (SC240) were treated with dimethyl sulfoxide (1%) (upper panels) or 25 μg/mL MBC (lower panels) for 20 min and then analysed for Wsc1p-GFP localization (left), mCherry-atb2 (middle) or Nomarski (right). (B) Cells expressing Mtl2p-GFP and mCherry-atb2 (SC242) were treated and analysed as above.|
|mbo3113-sup-0003-FigS3.tif||image/tif||4367K||Figure S3. The distribution of Rgf1p-GFP, Rgf2p-GFP and Rgf3p-GFP is not affected in strains deleted for wsc1+. (Upper panel) localization of Rgf1p-GFP in wild-type (PG40) and wsc1Δ cells (SC165). (Middle panel) localization of Rgf2p-GFP, in wild-type (HVP54 + p41X-Rgf2-GFP) and wsc1Δ cells (SC136+ p41X-Rgf2-GFP). (Bottom panel) localization of Rgf3p-GFP in wild-type (SC238) and wsc1Δ cells (SC198). Cells were grown to log phase in YES medium at 28ºC.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.