Exploration of Deinococcus-Thermus molecular diversity by novel group-specific PCR primers
Article first published online: 29 AUG 2013
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 5, pages 862–872, October 2013
How to Cite
MicrobiologyOpen 2013; 2(5): 862–872
- Issue published online: 8 OCT 2013
- Article first published online: 29 AUG 2013
- Manuscript Accepted: 5 JUL 2013
- Manuscript Revised: 4 JUL 2013
- Manuscript Received: 21 MAR 2013
- Provence-Alpes-Côte d'Azur regional council
- Deinococcus-Thermus ;
- group-specific primers;
- molecular diversity
The deeply branching Deinococcus-Thermus lineage is recognized as one of the most extremophilic phylum of bacteria. In previous studies, the presence of Deinococcus-related bacteria in the hot arid Tunisian desert of Tataouine was demonstrated through combined molecular and culture-based approaches. Similarly, Thermus-related bacteria have been detected in Tunisian geothermal springs. The present work was conducted to explore the molecular diversity within the Deinococcus-Thermus phylum in these extreme environments. A set of specific primers was designed in silico on the basis of 16S rRNA gene sequences, validated for the specific detection of reference strains, and used for the polymerase chain reaction (PCR) amplification of metagenomic DNA retrieved from the Tataouine desert sand and Tunisian hot spring water samples. These analyses have revealed the presence of previously undescribed Deinococcus-Thermus bacterial sequences within these extreme environments. The primers designed in this study thus represent a powerful tool for the rapid detection of Deinococcus-Thermus in environmental samples and could also be applicable to clarify the biogeography of the Deinococcus-Thermus phylum.