Fitness and proteome changes accompanying the development of erythromycin resistance in a population of Escherichia coli grown in continuous culture
Article first published online: 28 AUG 2013
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 5, pages 841–852, October 2013
How to Cite
MicrobiologyOpen 2013; 2(5): 841–852
- Issue published online: 8 OCT 2013
- Article first published online: 28 AUG 2013
- Manuscript Accepted: 16 JUL 2013
- Manuscript Revised: 4 JUL 2013
- Manuscript Received: 7 MAY 2013
- Grant Agency of the Academy of Sciences of the Czech Republic. Grant Number: IAA500200913
- Czech Academy of Sciences. Grant Number: 61388971
- Continuous cultivation system;
- Escherichia coli ;
We studied the impact of a sublethal concentration of erythromycin on the fitness and proteome of a continuously cultivated population of Escherichia coli. The development of resistance to erythromycin in the population was followed over time by the gradient plate method and minimum inhibitory concentration (MIC) measurements. We measured the growth rate, standardized efficiency of synthesis of radiolabeled proteins, and translation accuracy of the system. The proteome changes were followed over time in two parallel experiments that differed in the presence or absence of erythromycin. A comparison of the proteomes at each time point (43, 68, and 103 h) revealed a group of unique proteins differing in expression. From all 35 proteins differing throughout the cultivation, only three were common to more than one time point. In the final population, a significant proportion of upregulated proteins was localized to the outer or inner cytoplasmic membranes or to the periplasmic space. In a population growing for more than 100 generations in the presence of antibiotic, erythromycin-resistant bacterial clones with improved fitness in comparison to early resistant culture predominated. This phenomenon was accompanied by distinct changes in protein expression during a stepwise, population-based development of erythromycin resistance.