Fitness and proteome changes accompanying the development of erythromycin resistance in a population of Escherichia coli grown in continuous culture
Article first published online: 28 AUG 2013
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 5, pages 841–852, October 2013
How to Cite
MicrobiologyOpen 2013; 2(5): 841–852
- Issue published online: 8 OCT 2013
- Article first published online: 28 AUG 2013
- Manuscript Accepted: 16 JUL 2013
- Manuscript Revised: 4 JUL 2013
- Manuscript Received: 7 MAY 2013
- Grant Agency of the Academy of Sciences of the Czech Republic. Grant Number: IAA500200913
- Czech Academy of Sciences. Grant Number: 61388971
|mbo3121-sup-0001-FigS1.tif||image/tif||2704K||Figure S1. Continuous cultivation equipment set up with two glass fermenters.|
|mbo3121-sup-0002-FigS2.eps||image/eps||6225K||Figure S2. Graphical presentation of protein expression differences for all PDQuest software selected protein spots from 43, 68 and 103 h samples of E. coli culture continuously grown in absence (green) and presence (red) of erythromycin (10 μg/mL). The spot density values in arbitrary units are normalized to EF-Tu concentration as a measure of the overall translation system efficiency.|
|mbo3121-sup-0003-TableS1.eps||image/eps||1993K||Table S1. Erythromycin resistance represented by MIC values for 0, 50, 103, and 120 h samples of E. coli culture continuously grown in the absence (Control) and presence (Ery) of erythromycin (10 μg/mL). MIC values were determined by microdilution assay performed in 96-well plates in a final volume of 100 μL. Inhibition of proliferation was determined by optical density measurements (600 nm) after overnight incubation at 37°C.|
|mbo3121-sup-0004-TableS2.pdf||application/PDF||154K||Table S2. Complete list of MALDI functionally classified proteins in all the spots differentially selected by PDQuest software from comparison of 2-DE proteomes from erythromycin and control experiment.|
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