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mbo3135-sup-0001-FigS1.tifimage/tif4114KFigure S1. Rarefaction curves for the OTU (97% similarity) sequences of 16S rRNA genes from X. muta and seawater based on observed species estimates (A). Water samples are abbreviated H20 and sponge samples are abbreviated Xm. Samples are further labeled by location (FL, Florida Keys; LC, Little Cayman; LSI, Bahamas) and replicate number (1–3). Also shown are the average rank abundance curves based on the number of sequences in each OTU for X. muta (“Sponge”) and for seawater (“Seawater”) (B).
mbo3135-sup-0002-FigS2.tifimage/tif4198KFigure S2. The ten most abundant OTUs that are unique to each location. For LC there was only one unique OTU. “Bacteria” indicates that an unidentified bacteria was the closest match. OTUs are ordered in decreasing abundance for each location and taxonomic names shown are the lowest taxonomic assignment by RDP. Locations are represented by colors shown by the legend and a second legend provides a scale for OTU abundance. The OTU number (identity) is shown in parentheses for each OTU, and OTU abundance refers to the number of sequences within that OTU. FL, Florida Keys; LC, Little Cayman; LSI, Bahamas.
mbo3135-sup-0003-FigS3.tifimage/tif5855KFigure S3. The ten most abundant OTUs that are unique to individual sponges from LC. OTUs are ordered in decreasing abundance for each individual and taxonomic names shown are the lowest taxonomic assignment by RDP. “Bacteria” indicates that an unidentified bacteria was the closest match. Individual sponges are represented by colors shown by the legend and a second legend provides a scale for OTU abundance. The OTU number (identity) is shown in parentheses for each OTU, and OTU abundance refers to the number of sequences within that OTU. FL, Florida Keys; LC, Little Cayman; LSI, Bahamas.
mbo3135-sup-0004-TableS1.docxWord document89KTable S1. Results of the OTU category significance test in QIIME, the corrected P-value, means at each location, and a consensus lineage is given for each significant OTU (listed by OTU number).

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