The Yersinia enterocolitica Ysa type III secretion system is expressed during infections both in vitro and in vivo
Version of Record online: 24 OCT 2013
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 6, pages 962–975, December 2013
How to Cite
MicrobiologyOpen 2013; 2(6): 962–975
- Issue online: 6 DEC 2013
- Version of Record online: 24 OCT 2013
- Manuscript Revised: 12 SEP 2013
- Manuscript Accepted: 12 SEP 2013
- Manuscript Received: 16 JUL 2013
- National Institutes of Health. Grant Numbers: R21 AI156042, T32 AI60555
- Sandia National Laboratories' LDRD. Grant Numbers: 165767, 171001
|mbo3136-sup-0001-FigS1.tif||image/tif||656K||Figure S1. The organization of the Ysa locus. The Ysa locus is located within the plasticity zone, a chromosomal region unique to Y. enterocolitica biovar 1B, which encodes several virulence factors. The locus consists of 29 genes with the indicated functions. Note that yspP, a gene encoding a secreted effector of the Ysa T3SS, is located outside of the Ysa locus.|
|mbo3136-sup-0002-FigS2.pdf||application/PDF||48K||Figure S2. GFP intensity reflects gfp expression. Bacteria were cultured under a fliC inducing temperature (26°C) and allowed to grow for 11 h, with samples being taken every hour for analysis of mean GFP intensity. After 6 h of growth, an aliquot was subcultured into a non-inducing condition (37°C). (A). The fliC::gfp reporter strain exhibits an immediate and permanent drop in mean GFP intensity upon switching to the non-inducing condition. Note that plasmid pSRB1, the fliC::gfp reporter used in this study, carries the fliC promoter of Salmonella enterica Serovar Typhimurium. (B). The promoterless gfp reporter serves as a negative control, with negligible GFP expression regardless of growth condition. Note the y-axis scale difference between panels (A and B).|
Table S1. Bacterial strains and plasmids used in this study.
Table S2. Primers used in this study.
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