mbo3136-sup-0001-FigS1.tifimage/tif656KFigure S1. The organization of the Ysa locus. The Ysa locus is located within the plasticity zone, a chromosomal region unique to Y. enterocolitica biovar 1B, which encodes several virulence factors. The locus consists of 29 genes with the indicated functions. Note that yspP, a gene encoding a secreted effector of the Ysa T3SS, is located outside of the Ysa locus.
mbo3136-sup-0002-FigS2.pdfapplication/PDF48KFigure S2. GFP intensity reflects gfp expression. Bacteria were cultured under a fliC inducing temperature (26°C) and allowed to grow for 11 h, with samples being taken every hour for analysis of mean GFP intensity. After 6 h of growth, an aliquot was subcultured into a non-inducing condition (37°C). (A). The fliC::gfp reporter strain exhibits an immediate and permanent drop in mean GFP intensity upon switching to the non-inducing condition. Note that plasmid pSRB1, the fliC::gfp reporter used in this study, carries the fliC promoter of Salmonella enterica Serovar Typhimurium. (B). The promoterless gfp reporter serves as a negative control, with negligible GFP expression regardless of growth condition. Note the y-axis scale difference between panels (A and B).

Table S1. Bacterial strains and plasmids used in this study.

Table S2. Primers used in this study.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.