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mbo3136-sup-0001-FigS1.tifimage/tif656KFigure S1. The organization of the Ysa locus. The Ysa locus is located within the plasticity zone, a chromosomal region unique to Y. enterocolitica biovar 1B, which encodes several virulence factors. The locus consists of 29 genes with the indicated functions. Note that yspP, a gene encoding a secreted effector of the Ysa T3SS, is located outside of the Ysa locus.
mbo3136-sup-0002-FigS2.pdfapplication/PDF48KFigure S2. GFP intensity reflects gfp expression. Bacteria were cultured under a fliC inducing temperature (26°C) and allowed to grow for 11 h, with samples being taken every hour for analysis of mean GFP intensity. After 6 h of growth, an aliquot was subcultured into a non-inducing condition (37°C). (A). The fliC::gfp reporter strain exhibits an immediate and permanent drop in mean GFP intensity upon switching to the non-inducing condition. Note that plasmid pSRB1, the fliC::gfp reporter used in this study, carries the fliC promoter of Salmonella enterica Serovar Typhimurium. (B). The promoterless gfp reporter serves as a negative control, with negligible GFP expression regardless of growth condition. Note the y-axis scale difference between panels (A and B).
mbo3136-sup-00023-TableS1-S2.pdfapplication/PDF95K

Table S1. Bacterial strains and plasmids used in this study.

Table S2. Primers used in this study.

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