Contributed equally to the work.
Canonical and non-canonical EcfG sigma factors control the general stress response in Rhizobium etli
Version of Record online: 28 OCT 2013
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 6, pages 976–987, December 2013
How to Cite
MicrobiologyOpen 2013; 2(6): 976–987
- Issue online: 6 DEC 2013
- Version of Record online: 28 OCT 2013
- Manuscript Accepted: 23 SEP 2013
- Manuscript Revised: 16 SEP 2013
- Manuscript Received: 27 JUL 2013
- Belgian Science Policy Office
- Research Council of the KU Leuven. Grant Number: GOA/011/2008
- Fund for Scientific Research-Flanders. Grant Number: G.0412.10
|mbo3137-sup-0001-FigS1.pdf||application/PDF||178K||Figure S1. RT-qPCR validation of the microarray data. Expression of 13 genes was determined using RT-qPCR for wild type, ΔecfG1, ΔecfG2, and ΔecfG1ΔecfG2. The log2 transformed mean values of three replicates were used to report three different fold changes for each gene (Y-axis) compared to the respective microarray fold changes (X-axis). Black squares represent wild type versus ΔecfG1; light gray diamonds wild type versus ΔecfG2 and dark gray dots wild type versus ΔecfG1ΔecfG2|
|mbo3137-sup-0002-FigS2.pdf||application/PDF||15K||Figure S2. σEcfG1-, σEcfG2- and σEcfG1-σEcfG2-dependent ncRNA expression. Venn diagram of all differentially expressed ncRNAs in ΔecfG1, ΔecfG2, and ΔecfG1ΔecfG2 strains compared to the wild-type strain R. etli CFN42. Upward- and downward-oriented arrows indicate gene induction and repression, respectively.|
|mbo3137-sup-0003-TableS1.pdf||application/PDF||38K||Table S1. Bacterial strains and plasmids used in this study.|
|mbo3137-sup-0004-TableS2.pdf||application/PDF||40K||Table S2. Primers used in this study.|
|mbo3137-sup-0005-TableS3.pdf||application/PDF||34K||Table S3. Distribution of σEcfG sigma factors in completely sequenced α-proteobacterial genomes. Data retrieved from MiST database (http://mistdb.com; (Ulrich and Zhulin 2010) on 29 November 2012.|
|mbo3137-sup-0006-TableS4.pdf||application/PDF||55K||Table S4. The differentially expressed genes and ncRNAs in ΔecfG1, ΔecfG2, and ΔecfG1ΔecfG2 compared to the wild type.|
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