Members of the uncultured bacterial candidate division WWE1 are implicated in anaerobic digestion of cellulose
Article first published online: 5 FEB 2014
© 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 3, Issue 2, pages 157–167, April 2014
How to Cite
MicrobiologyOpen 2014; 3(2): 157–167
- Issue published online: 9 APR 2014
- Article first published online: 5 FEB 2014
- Manuscript Accepted: 28 OCT 2013
- Manuscript Revised: 21 OCT 2013
- Manuscript Received: 31 MAY 2013
- CMCU. Grant Number: 07G 0801
- Service de coopération et d'action culturelle, Ministère des affaires étrangères
- Cellulose anaerobic digestion;
- FISH ;
- SIMSISH ;
- SIP ;
- WWE1 candidate division
Clones of the WWE1 (Waste Water of Evry 1) candidate division were retrieved during the exploration of the bacterial diversity of an anaerobic mesophilic (35 ± 0.5°C) digester. In order to investigate the metabolic function of WWE1 members, a 16S rRNA gene -based stable isotope probing (SIP) method was used. Eighty-seven percent of 16S r rRNA gene sequences affiliated to WWE1 candidate division were retrieved in a clone library obtained after polymerase chain reaction (PCR) amplification of enriched DNA fraction from anaerobic municipal solid waste samples incubated with 13C-cellulose, at the end of the incubation (day 63) using a Pla46F-1390R primer pair. The design of a specific WWE1 probe associated with the fluorescence in situ hybridization (FISH) technique corroborated the abundant representation of WWE1 members in our 13C-cellulose incubations. Secondary ion mass spectrometry–in situ hybridization (SIMSISH) using an iodine-labeled oligonucleotide probe combined with high-resolution nanometer-scale SIMS (NanoSIMS) observation confirmed the isotopic enrichment of members of WWE1 candidate division. The 13C apparent isotopic composition of hybridized WWE1 cells reached the value of about 40% early during the cellulose degradation process, suggesting that these bacteria play a role either in an extracellular cellulose hydrolysis process and/or in the uptake fermentation products.