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mbo3186-sup-0001-FigS1.tifimage/tif2347KFigure S1. Yeast cells presenting chlamydial Pmps on their cell surface. (A) Detection of Aga2 and Aga2-Pmp fusion proteins on the yeast cell surface. Green fluorescent yeast cells expressing Aga2 and Aga2–Pmp fusion proteins were fixed and stained with an anti-V5 antibody (red) against the C-terminal V5-Tag. Top panel: Phase contrast microscopy. Bottom panel: Fluorescence microscopy. (B) Western blot analysis of Aga2 and Aga2-Pmp fusion proteins. Total protein extracts from yeast strains expressing Aga2 or Aga2–Pmp fusion proteins were digested with α-mannosidase to remove Aga2 O-glycosylation, resolved by SDS-PAGE and probed with an anti-His antibody. Protein size markers (in kDa) are indicated to the left of each blot.
mbo3186-sup-0002-FigS2.tifimage/tif538KFigure S2. Coating efficiency of latex beads. Western blot analysis of recombinant proteins coated on 2 × 107 latex beads (adhesion data shown in Fig. 1C). Proteins were removed from the bead surface by SDS-loading buffer and DTT, resolved by SDS-PAGE, and probed with an antibody against the N-terminal His tag. Lane M, molecular mass marker.
mbo3186-sup-0003-TableS1.pdfapplication/PDF248KTable S1. List of oligonucleotides used for plasmid construction.

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