mbo3186-sup-0001-FigS1.tifimage/tif2347KFigure S1. Yeast cells presenting chlamydial Pmps on their cell surface. (A) Detection of Aga2 and Aga2-Pmp fusion proteins on the yeast cell surface. Green fluorescent yeast cells expressing Aga2 and Aga2–Pmp fusion proteins were fixed and stained with an anti-V5 antibody (red) against the C-terminal V5-Tag. Top panel: Phase contrast microscopy. Bottom panel: Fluorescence microscopy. (B) Western blot analysis of Aga2 and Aga2-Pmp fusion proteins. Total protein extracts from yeast strains expressing Aga2 or Aga2–Pmp fusion proteins were digested with α-mannosidase to remove Aga2 O-glycosylation, resolved by SDS-PAGE and probed with an anti-His antibody. Protein size markers (in kDa) are indicated to the left of each blot.
mbo3186-sup-0002-FigS2.tifimage/tif538KFigure S2. Coating efficiency of latex beads. Western blot analysis of recombinant proteins coated on 2 × 107 latex beads (adhesion data shown in Fig. 1C). Proteins were removed from the bead surface by SDS-loading buffer and DTT, resolved by SDS-PAGE, and probed with an antibody against the N-terminal His tag. Lane M, molecular mass marker.
mbo3186-sup-0003-TableS1.pdfapplication/PDF248KTable S1. List of oligonucleotides used for plasmid construction.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.