Affinity maturation of Cry1Aa toxin to the Bombyx mori cadherin-like receptor by directed evolution based on phage display and biopanning selections of domain II loop 2 mutant toxins
Version of Record online: 16 JUL 2014
© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 3, Issue 4, pages 568–577, August 2014
How to Cite
MicrobiologyOpen 2014; 3(4): 568–577
- Issue online: 14 AUG 2014
- Version of Record online: 16 JUL 2014
- Manuscript Accepted: 26 MAY 2014
- Manuscript Revised: 12 MAY 2014
- Manuscript Received: 13 MAR 2014
- Ministry of Education, Culture, Sports, Science, and Technology of Japan. Grant Number: 24310054
Figure S1. SDS-PAGE electrophoretogram of trypsin-activated wild-type toxin, 371WGLA375, and 371WPHH375. Protoxins were activated by 0.5 mg−1 mL−1 trypsin in 20 mmol L−1 Tris-HCl, pH 8.3 containing 150 mmol L−1 NaCl for 2 h at 37°C in the DEAE column. Lane 1: wild-type toxin, lane 2: 371WGLA375, lane 3: 371WPHH375.
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