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Figure S1. SDS-PAGE electrophoretogram of trypsin-activated wild-type toxin, 371WGLA375, and 371WPHH375. Protoxins were activated by 0.5 mg−1 mL−1 trypsin in 20 mmol L−1 Tris-HCl, pH 8.3 containing 150 mmol L−1 NaCl for 2 h at 37°C in the DEAE column. Lane 1: wild-type toxin, lane 2: 371WGLA375, lane 3: 371WPHH375.

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