Both authors contributed equally to this work.
Biological effects of paenilamicin, a secondary metabolite antibiotic produced by the honey bee pathogenic bacterium Paenibacillus larvae
Version of Record online: 16 JUL 2014
© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 3, Issue 5, pages 642–656, October 2014
How to Cite
MicrobiologyOpen 2014; 3(5): 642–656
- Issue online: 9 OCT 2014
- Version of Record online: 16 JUL 2014
- Manuscript Accepted: 16 JUN 2014
- Manuscript Revised: 3 JUN 2014
- Manuscript Received: 10 FEB 2014
- Ministries for Agriculture
- German Research Foundation
- Cluster of Excellence “Unifying Concepts in Catalysis”
Figure S1. Comparison of LC-ESI-Orbitrap-MS profiling of metabolites present in the crude supernatant. (A) Total ion chromatogram (TIC) of P. larvae DSM25430 wt and (B) total ion chromatogram (TIC) of P. larvae DSM25430 ∆pamA. Paenilamicin could not be detected directly from crude supernatants due to early elution in the dead volume and ion suppression. The secondary metabolite sevadicin is indicated with a red arrow.
Figure S2. MS/MS fragmentation studies on paenilamicin by HPLC-ESI-triple quadrupole MS from P. larvae secretome after sample workup. (A) MS/MS fragmentation spectrum with [M + 2H]2+ = 505.4 as precursor ion (♦). (B) MS/MS fragmentation spectrum with [M + 2H]2+ = 519.4 as precursor ion (♦).
Figure S3. HPLC UV analytics of pure paenilamicin. (A) Chromatogram of pure paenilamicin monitored at λ = 214 nm. (B) Chromatogram of a blank measurement (H2O) monitored at λ = 214 nm. Paenilamicin shows practically no retention at the C18 column.
Table S1. Homology analysis of the pam gene cluster products.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.