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Figure S1. Southern blot analysis of gene inactivation mutants in M. oryzae with HIK-gene-specific probes. Genomic DNA of M. oryzae strain 70-15 and the mutants were isolated and restricted with corresponding restriction enzymes. The probes which we used for hybridization with the genomic DNA of the wild-type strain and the corresponding mutant strains were always identical. “*” marked the position of the hybridization of the probes.

Figure S2. Protein domain scheme of the sequences which were used for the phylogenetic analysis of the two-component hybrid histidine kinases of fungi. Bf (Botryotinia fuckeliana), Ca (Candida albicans), Ch (Cochliobolus heterostrophus), Ec (Escherichia coli), En (Emericella nidulans), Gm (Gibberella moniliformis), Mg (Mycosphaerella graminicola), Mo (Magnaporthe oryzae), Nc (Neurospora crassa), Sc (Saccharomyces cerevisiae). HisKA, histidine kinase domain; REC, regulatory domain; HATPase, histidine-ATPase domain; ATPase, ATPase domain; PKc, protein kinase domain; GAF, GAF domain; HAMP, HAMP domain; PAS, PAS domain; PASF, PASF domain; PAC, PAC domain; PHY, phytochrome domain; TM, transmembrane domain.

Figure S3. Vegetative growth of the Magnaporthe oryzae wild-type strain 70-15 and the HIK mutants on complete medium. The fungal strains were grown on complete medium (CM) with additional stress inducing agents NaCl, sorbitol, NaNO2, CoCl2, or CuSO4 for 10 days at 26°C.

Figure S4. Vegetative growth of the Magnaporthe oryzae wild-type strain 70-15 and the HIK mutants on minimal medium. The fungal strains were grown on minimal medium (MM) with additional stress inducing agents NaCl, sorbitol, NaNO2, CoCl2, CuSO4, or H2O2 for 10 days at 26°C.

Table S1. List of oligonucleotides used in this study.

Table S2. Vegetative growth of the Magnaporthe oryzae wild-type strain 70-15 and the HIK mutants.

Table S3. GeneBank accession numbers or the gene name from the Magnaporthe comparative database of the two-component hybrid histidine kinases used for the phylogenetic analysis.

Data S1. Strategies of Inactivating Genes Within the Magnaporthe oryzae genome.

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