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MicrobiologyOpen

Cover image for Vol. 1 Issue 4

December 2012

Volume 1, Issue 4

Pages i–ii, 349–513

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. Original Research
    4. Review
    1. You have full text access to this OnlineOpen article
      Issue Information (pages i–ii)

      Article first published online: 12 DEC 2012 | DOI: 10.1002/mbo3.15

  2. Original Research

    1. Top of page
    2. Issue Information
    3. Original Research
    4. Review
    1. You have full text access to this OnlineOpen article
      Reversible oxygen-tolerant hydrogenase carried by free-living N2-fixing bacteria isolated from the rhizospheres of rice, maize, and wheat (pages 349–361)

      Philippe Roumagnac, Pierre Richaud, Mohamed Barakat, Philippe Ortet, Marie-Anne Roncato, Thierry Heulin, Gilles Peltier, Wafa Achouak and Laurent Cournac

      Article first published online: 12 SEP 2012 | DOI: 10.1002/mbo3.37

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      We present here the result of screening for hydrogenase activity of hundreds of bacterial strains collected from several ecosystems. We more specifically highlight the presence of a relatively oxygen-tolerant Hyc-type hydrogenase in a family of Enterobacteria, show some specificities of their sequences and discuss potential ecophysiological implications and biotechnological interest.

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      Seasonal variability of prevalence and occurrence of multiple infections shape the population structure of Crithidia bombi, an intestinal parasite of bumblebees (Bombus spp.) (pages 362–372)

      Mario Popp, Silvio Erler and H. Michael G. Lattorff

      Article first published online: 23 SEP 2012 | DOI: 10.1002/mbo3.35

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      The manuscript presents the study of the prevalence and the intensity of infection of the gut parasite Crithidia bombi in temporally distinct samples of two common bumblebee species, Bombus terrestris and Bombus lapidarius and its effects on the population genetic structure of the parasite. Prevalence of the parasite was used as a measure of transmission within and between the two host populations. Analysis of microsatellite markers were used to study the influence of seasonal differences in prevalence and the occurrence of multiple infections on the population structure of the parasite. Furthermore, the genetic composition of parasite population changes drastically during the course of the season.

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      Immunomodulatory effects of heat-killed Enterococcus faecalis TH10 on murine macrophage cells (pages 373–380)

      Tomohiro Itoh, Yasuyoshi Miyake, Ayumi Onda, Junko Kubo, Masashi Ando, Yasuyuki Tsukamasa and Muneaki Takahata

      Article first published online: 30 SEP 2012 | DOI: 10.1002/mbo3.41

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      The resultant TLR2-TLR1/6 pathway activation by hk-TH10 induces the expression of iNOS and COX-2. hk-TH10 may be useful in foods for the facilitation of host immunomodulation.

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      A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli (pages 381–394)

      Tomohiro Shimada, Yasunori Katayama, Shuichi Kawakita, Hiroshi Ogasawara, Masahiro Nakano, Kaneyoshi Yamamoto and Akira Ishihama

      Article first published online: 8 OCT 2012 | DOI: 10.1002/mbo3.42

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      The csgD gene encoding the master regulator of biofilm formation carries the most complex promoter in Escherichia coli. More than 10 transcription factors including the newly identified RcdA are involved in regulation.

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      Polyester production by halophilic and halotolerant bacterial strains obtained from mangrove soil samples located in Northern Vietnam (pages 395–406)

      Doan Van-Thuoc, Tran Huu-Phong, Nguyen Thi-Binh, Nguyen Thi-Tho, Duong Minh-Lam and Jorge Quillaguamán

      Article first published online: 11 OCT 2012 | DOI: 10.1002/mbo3.44

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      Biopolyester production by halophilic and halotolerant bacteria isolated from Vietnam.

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      The differential susceptibility of spores from virulent and attenuated Bacillus anthracis strains to aldehyde- and hypochlorite-based disinfectants (pages 407–414)

      Jordon K. March, Marissa N. Cohen, James M. Lindsey, D. A. Millar, Chinn-Woan Lowe, Annette J. Bunnell, Kim L. O'Neill, G. Bruce Schaalje and Richard A. Robison

      Article first published online: 11 OCT 2012 | DOI: 10.1002/mbo3.45

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      This study evaluated the inactivation kinetics of aldehyde- and hypochlorite-based disinfectants against spores from virulent and attenuated Bacillus anthracis. A novel statistical model was used to determine 4-log10 reduction times for each strain/disinfectant combination. Spores from virulent and attenuated strains did display significantly different susceptibilities to the different disinfectants.

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      Bacterial diversity in different regions of gastrointestinal tract of Giant African Snail (Achatina fulica) (pages 415–426)

      Kiran D. Pawar, Sunil Banskar, Shailendra D. Rane, Shakti S. Charan, Girish J. Kulkarni, Shailesh S. Sawant, Hemant V. Ghate, Milind S. Patole and Yogesh S. Shouche

      Article first published online: 19 OCT 2012 | DOI: 10.1002/mbo3.38

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      Bacterial diversity in the different regions of gastrointestinal tract of Giant African Snail, Achatina fulica by culture-independent and culture-dependent methods, has been studied.

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      Survival of enterohemorrhagic Escherichia coli in the presence of Acanthamoeba castellanii and its dependence on Pho regulon (pages 427–437)

      Samuel Mohammed Chekabab, France Daigle, Steve J. Charette, Charles M. Dozois and Josée Harel

      Article first published online: 30 OCT 2012 | DOI: 10.1002/mbo3.40

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      The numbers of EHEC were significantly higher when cultured with amoebae than without, and less EHEC shifted into a viable but non-culturable state in the presence of amoebae. Using several mutants, we observed that the Pho regulon is required for EHEC growth when co-cultured with amoebae. In contrast, the Shiga toxins (Stx) were not involved in this association phenotype.

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      Assessment of the relevance of the antibiotic 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine from Pantoea agglomerans biological control strains against bacterial plant pathogens (pages 438–449)

      Ulrike F. Sammer, Katharina Reiher, Dieter Spiteller, Annette Wensing and Beate Völksch

      Article first published online: 30 OCT 2012 | DOI: 10.1002/mbo3.43

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      The epiphyte Pantoea agglomerans 48b/90 (Pa48b) is a promising biocontrol strain against economically important bacterial pathogens such as Erwinia amylovora. Strain Pa48b produces the broad-spectrum antibiotic 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine (APV) in a temperature-dependent manner. An APV-negative mutant still suppressed the E. amylovora population and fire blight disease symptoms in apple blossom experiments under greenhouse conditions, but was inferior to the Pa48b wild-type indicating the influence of APV in the antagonism. In plant experiments with the soybean pathogen Pseudomonas syringae pv. glycinea both, Pa48b and the APV-negative mutant, successfully suppressed the pathogen. Our results demonstrate that the P. agglomerans strain Pa48b is an efficient biocontrol organism against plant pathogens, and we prove its ability for fast colonization of plant surfaces over a wide temperature range.

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      Depletion of acidic phospholipids influences chromosomal replication in Escherichia coli (pages 450–466)

      Nicholas Fingland, Ingvild Flåtten, Christopher D. Downey, Solveig Fossum-Raunehaug, Kirsten Skarstad and Elliott Crooke

      Article first published online: 16 NOV 2012 | DOI: 10.1002/mbo3.46

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      Depletion of acidic phospholipids in Escherichia coli leads to inhibited chromosomal replication and a decrease in cell mass, followed by growth arrest. Restoration of acidic phospholipid synthesis results in a resumption of DNA replication prior to restored growth, indicating a possible cell-cycle-specific growth arrest had occurred with the earlier loss of acidic phospholipids.

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      Diversity of symbioses between chemosynthetic bacteria and metazoans at the Guiness cold seep site (Gulf of Guinea, West Africa) (pages 467–480)

      Sébastien Duperron, Clara F. Rodrigues, Nelly Léger, Kamil Szafranski, Carole Decker, Karine Olu and Sylvie M. Gaudron

      Article first published online: 21 NOV 2012 | DOI: 10.1002/mbo3.47

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      Four bivalve and one annelid species were collected in the recently discovered Guiness cold seep site in the Gulf of Guinea. In this article, we show that sulfur-oxidizing γ-proteobacteria occur as symbionts in the gills of bivalves, and in the trophosome of the annelid; these bacteria are related to various symbionts associated with deep-sea metazoans. These new data, the first available from this particular site located in the equatorial east Atlantic, provides new insights into the biogeography of chemosynthetic symbioses.

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      Molecular identification in monophasic and nonmotile variants of Salmonella enterica serovar Typhimurium (pages 481–489)

      M. Bugarel, M.-L. Vignaud, F. Moury, P. Fach and A. Brisabois

      Article first published online: 26 NOV 2012 | DOI: 10.1002/mbo3.39

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      The manuscript describes a genotypic approach for the characterization of the emerging monophasic S.1,4,[5],12:i:- of Salmonella serovar Typhimurium and the other monophasic and nonmotile-related variants. This study could be of great contributes to enhance the identification and surveillance of Salmonella strains especially for epidemiological investigations.

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      Extragenic suppressor mutations that restore twitching motility to fimL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels (pages 490–501)

      Laura M. Nolan, Scott A. Beatson, Larry Croft, Peter M. Jones, Anthony M. George, John S. Mattick, Lynne Turnbull and Cynthia B. Whitchurch

      Article first published online: 28 NOV 2012 | DOI: 10.1002/mbo3.49

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      Pseudomonas aeruginosa fimL mutants, which usually demonstrate defective twitching motility, frequently revert to a wild-type twitching-motility phenotype presumably via the acquisition of an extragenic suppressor mutation(s). In this study, we have characterized five independent fimL twitching-motility revertants and have determined that all have increased intracellular cAMP levels compared with the parent fimL mutant. Whole-genome sequencing revealed that only one of these fimL revertants has acquired a loss-of-function mutation in cpdA that accounts for the elevated levels of intracellular cAMP.

  3. Review

    1. Top of page
    2. Issue Information
    3. Original Research
    4. Review
    1. You have full text access to this OnlineOpen article
      The regulator HlyU, the repeat-in-toxin gene rtxA1, and their roles in the pathogenesis of Vibrio vulnificus infections (pages 502–513)

      Moqing Liu and Jorge H. Crosa

      Article first published online: 28 NOV 2012 | DOI: 10.1002/mbo3.48

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      HlyU is a master regulator that plays an essential role in the virulence of the human pathogen Vibrio vulnificus. One of the most noteworthy characteristics of HlyU regulation in this organism is its positive control of the expression of the repeat-in-toxin toxin (RtxA1) gene. In this work, we reviewed the latest studies of RtxA1 in this bacterium and highlight the mechanism of gene regulation of rtxA1 expression by HlyU under a broader gene regulatory network.

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