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MicrobiologyOpen

Cover image for Vol. 2 Issue 3

June 2013

Volume 2, Issue 3

Pages i–ii, 365–539

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. Original Research
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      Issue Information (pages i–ii)

      Version of Record online: 7 JUN 2013 | DOI: 10.1002/mbo3.97

  2. Original Research

    1. Top of page
    2. Issue Information
    3. Original Research
    1. You have full text access to this OnlineOpen article
      Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation (pages 365–382)

      Morten S. Dueholm, Mads T. Søndergaard, Martin Nilsson, Gunna Christiansen, Allan Stensballe, Michael T. Overgaard, Michael Givskov, Tim Tolker-Nielsen, Daniel E. Otzen and Per H. Nielsen

      Version of Record online: 18 MAR 2013 | DOI: 10.1002/mbo3.81

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      Functional amyloids in Pseudomonas (Fap) are so far only described for a single environmental isolate, termed Pseudomonas fluorescens UK4. Here, we show that medical and technical important Pseudomonas strains, including P. aeruginosa PAO1, have the genetical capacity to produce Fap. Expression of Fap triggers cell autoaggregation and speed up biofilm formation.

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      Identification of an O-antigen chain length regulator, WzzP, in Porphyromonas gingivalis (pages 383–401)

      Mikio Shoji, Hideharu Yukitake, Keiko Sato, Yasuko Shibata, Mariko Naito, Joseph Aduse-Opoku, Yoshimitsu Abiko, Michael A. Curtis and Koji Nakayama

      Version of Record online: 19 MAR 2013 | DOI: 10.1002/mbo3.84

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      We found a novel Porphyromonas gingivalis pigment-less mutant by transposon mutagenesis: the gene responsible was the PGN_2005 gene of strain ATCC 33277. Based on the analysis of the LPS pattern of the PGN_2005 mutant, the location and structure of the PGN_2005 protein and complementation of an Escherichia coli wzz mutant by the PGN_2005 gene, we believe that the PGN_2005 protein is Wzz of P. gingivalis, designated WzzP. In addition, in this study, we determined that the PGN_1896 and PGN_1233 proteins and the PGN_1033 protein appear to be WbaP homolog proteins and a Wzx homolog protein, respectively.

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      Pyrosequencing assessment of prokaryotic and eukaryotic diversity in biofilm communities from a French river (pages 402–414)

      Geneviève Bricheux, Loïc Morin, Gwenaël Le Moal, Gérard Coffe, Damien Balestrino, Nicolas Charbonnel, Jacques Bohatier and Christiane Forestier

      Version of Record online: 20 MAR 2013 | DOI: 10.1002/mbo3.80

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      We performed a comprehensive analysis of bacterial and eukaryotic communities of river biofilms by pyrosequencing rDNA amplicons. The data indicated a large diversity among prokaryotes with a predominance of Proteobacteria, together with an elevated number of rare sequences. Altogether, this would indicate an important photosynthetic activity within the biofilm.

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      A bipolar functionality of Q/N-rich proteins: Lsm4 amyloid causes clearance of yeast prions (pages 415–430)

      Keita Oishi, Hiroshi Kurahashi, Chan-Gi Pack, Yasushi Sako and Yoshikazu Nakamura

      Version of Record online: 20 MAR 2013 | DOI: 10.1002/mbo3.83

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      Here, we report that overexpression of a [PSI+]-inducible Q/N-rich protein, Lsm4, eliminates the three major prions [PSI+], [URE3], and [RNQ+]. In the course of the Lsm4-driven [PSI+] elimination, the [PSI+] aggregates undergo a size increase, which ultimately results in the formation of conspicuous foci in otherwise [psi]-like mother cells. We also found that the antiprion activity is a general property of [PSI+]-inducible factors. These data provoked a novel “unified” model that explains both prion induction and elimination by a single scheme.

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      EmbRS a new two-component system that inhibits biofilm formation and saves Rubrivivax gelatinosus from sinking (pages 431–446)

      Anne Soisig Steunou, Sylviane Liotenberg, Marie-Noêlle Soler, Romain Briandet, Valérie Barbe, Chantal Astier and Soufian Ouchane

      Version of Record online: 21 MAR 2013 | DOI: 10.1002/mbo3.82

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      Ability of the photosynthetic bacterium Rubrivivax gelatinosus to form biofilm. The EmbRS two-component system inhibits biofilm formation. Growth of the EmbRS mutant results in the formation of conspicuous bacterial veils around the toothpick scaffolds.

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      Characterization of Aphanizomenon ovalisporum amidinotransferase involved in cylindrospermopsin synthesis (pages 447–458)

      Ángel Barón-Sola, Miguel A. Gutiérrez-Villanueva, Francisca F. del Campo and Soledad Sanz-Alférez

      Version of Record online: 26 MAR 2013 | DOI: 10.1002/mbo3.78

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      The understanding of CYN synthesis would be crucial in control CYN production. Amidinotransferase (AMDT) seems to be the first enzyme involved in CYN synthesis. We have cloned and overexpressed the aoaA gene, which codifies for the AMDT, the constitutive CYN producer Aphanizomenon ovalisporum UAM-MAO. Purified AoaA was characterized, confirming that it is an l-arginine:glycine AMDT.

    7. You have full text access to this OnlineOpen article
      Truncation of type IV pilin induces mucoidy in Pseudomonas aeruginosa strain PAO579 (pages 459–470)

      T. Ryan Withers, F. Heath Damron, Yeshi Yin and Hongwei D. Yu

      Version of Record online: 27 MAR 2013 | DOI: 10.1002/mbo3.86

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      Schematic diagram of summarizing the induction of alginate production and mucoid conversion by PilA in Pseudomonas aeruginosa strain PAO579. The sigma factor RpoN is required for transcription of pilA108 and algW. PilA108 is transported to the periplasm where it activates the periplasmic protease AlgW which proteolytically degrades the anti-sigma factor MucA releasing the sequestered sigma factor AlgU.

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      A novel l-isoleucine-4′-dioxygenase and l-isoleucine dihydroxylation cascade in Pantoea ananatis (pages 471–481)

      Sergey V. Smirnov, Pavel M. Sokolov, Veronika A. Kotlyarova, Natalya N. Samsonova, Tomohiro Kodera, Masakazu Sugiyama, Takayoshi Torii, Makoto Hibi, Sakayu Shimizu, Kenzo Yokozeki and Jun Ogawa

      Version of Record online: 2 APR 2013 | DOI: 10.1002/mbo3.87

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      The genes encoding HilA and HilB from Pantoea ananatis AJ13355 were cloned and expressed in Escherichia coli. The culturing of E. coli cells expressing hilA (E. coli-HilA) or both hilA and hilB (E. coli-HilAB) in the presence of l-isoleucine resulted in the conversion of l-isoleucine into two novel biogenic compounds: l-4′-isoleucine and l-4,4′-dihydroxyisoleucine, respectively. In parallel, two novel enzymatic activities were detected in the crude cell lysates of the E. coli-HilA and E. coli-HilAB strains: l-isoleucine-4′-dioxygenase (HilA) and l-4′-hydroxyisoleucine-4-dioxygenase (HilB) activities, respectively.

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      Effect of fungicides on epiphytic yeasts associated with strawberry (pages 482–491)

      Jane Debode, Wendy Van Hemelrijck, Piet Creemers and Martine Maes

      Version of Record online: 4 APR 2013 | DOI: 10.1002/mbo3.85

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      Both conventional plating and denaturing gradient gel electrophoresis (DGGE) were used to analyse the effect of fungicides on epiphytic yeasts associated with strawberry. The results show that the main epiphytic yeasts are not affected by the fungicides and this could lead to an integrated use of epiphytic yeasts to control fungal diseases of strawberry.

    10. You have full text access to this OnlineOpen article
      Evaluating bacterial community structures in oil collected from the sea surface and sediment in the northern Gulf of Mexico after the Deepwater Horizon oil spill (pages 492–504)

      Zhanfei Liu and Jiqing Liu

      Version of Record online: 9 APR 2013 | DOI: 10.1002/mbo3.89

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      Phylogenetic analysis suggested that Erythrobacter, Rhodovulum, Stappia and Thalassospira of Alphaproteobacteria were the prevailing groups in the sea surface oil mousse collected during the Deepwater Horizon oil spill, and this pattern may relate to high temperatures and strong irradiance in surface Gulf waters. In the salt marshes mousse, Vibrio represented 57% of the total operational taxonomic units, suggesting that this indigenous genus is particularly responsive to the oil contamination in salt marshes. In contrast, bacterial community in oil-contaminated sediments near the well head was much diversified.

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      Roles of the Gac-Rsm pathway in the regulation of phenazine biosynthesis in Pseudomonas chlororaphis 30-84 (pages 505–524)

      Dongping Wang, Sung-Hee Lee, Candace Seeve, Jun Myoung Yu, Leland S. Pierson III and Elizabeth A. Pierson

      Version of Record online: 21 APR 2013 | DOI: 10.1002/mbo3.90

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      Transcriptomic analysis of a gacA mutant identified 771 genes including many novel genes in Pseudomonas chlororaphis 30-84. The rsmZ and rsmE genes were linked to phenazine production. Our results also showed that constitutive expression of the quorum sensing transcriptional activator phzR, with the addition of N-acyl-homoserine lactones did not rescue phenazine production in the gacA mutant, indicating the direct posttranscriptional control by Gac of the phenazine biosynthetic genes. Figure shows proposed model of Gac/Rsm regulation of phenazines in 30-84

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      Ubiquitination dynamics in the early-branching eukaryote Giardia intestinalis (pages 525–539)

      Carlos A. Niño, Jenny Chaparro, Paolo Soffientini, Simona Polo and Moises Wasserman

      Version of Record online: 23 APR 2013 | DOI: 10.1002/mbo3.88

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      Ubiquitation is an active and dynamic process in Giardia. Different types of ubiquitin modifications are present in this ancestral unicellular eukaryote and vary in terms of temporal and spatial distribution from trophozoites to cyst maturation. Using a proteomics approach, we identified around 200 high-confidence ubiquitinated candidates that vary their ubiquitination status during differentiation.

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