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MicrobiologyOpen

Cover image for Vol. 2 Issue 5

October 2013

Volume 2, Issue 5

Pages i–ii, 717–891

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. Original Research
    1. You have full text access to this Open Access content
      Issue Information (pages i–ii)

      Version of Record online: 8 OCT 2013 | DOI: 10.1002/mbo3.13

  2. Original Research

    1. Top of page
    2. Issue Information
    3. Original Research
    1. You have full text access to this OnlineOpen article
      Characterization of mineral phosphate solubilization traits from a barley rhizosphere soil functional metagenome (pages 717–724)

      Sagar Chhabra, Dina Brazil, John Morrissey, James I. Burke, Fergal O'Gara and David N. Dowling

      Version of Record online: 25 JUL 2013 | DOI: 10.1002/mbo3.110

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      A fosmid system was used to clone a barley rhizosphere soil metagenome, which was screened for mineral phosphate solubilization (MPS). The MPS clones were analyzed by 454 Roche sequencing and annotated. A number of genes/operons with homology to P uptake, regulatory and solubilization mechanisms were identified.

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      Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus (pages 725–742)

      Taira Matsuo, Koji Nakamura, Toshio Kodama, Taro Mikami, Hirotaka Hiyoshi, Tomofusa Tsuchiya, Wakano Ogawa and Teruo Kuroda

      Version of Record online: 27 JUL 2013 | DOI: 10.1002/mbo3.100

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      We characterized 12 RND-type multidrug efflux transporters in Vibrio parahaemolyticus. Deletion mutants of all twelve transporter genes showed higher sensitivity for several antibiotics and bile, and lower pathogenicity.

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      TusA (YhhP) and IscS are required for molybdenum cofactor-dependent base-analog detoxification (pages 743–755)

      Stanislav G. Kozmin, Elena I. Stepchenkova and Roel M. Schaaper

      Version of Record online: 29 JUL 2013 | DOI: 10.1002/mbo3.108

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      We show that Escherichia coli mutants deficient in the sulfurtransferase TusA or the cysteine desulfurase IscS are hypersensitive to the toxic effects of the adenine analog 6-N-hydroxylaminopurine (HAP). This sensitivity is similar to and epistatic with the HAP sensitivity of moa mutants, which defective in biosynthesis of the molybdenum cofactor (Moco). Our results suggest that TusA and IscS are critical for the insertion of the dithiolene sulfurs in Moco that coordinate the molybdenum atom.

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      Modeling the effect of copper availability on bacterial denitrification (pages 756–765)

      Hugh C. Woolfenden, Andrew J. Gates, Chris Bocking, Mark G. Blyth, David J. Richardson and Vincent Moulton

      Version of Record online: 30 JUL 2013 | DOI: 10.1002/mbo3.111

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      We have developed a mathematical model for the denitrification pathway based on existing experimental results, Michaelis–Menten kinetics and experimentally obtained kinetic constants. This is the first such model to incorporate the copper concentration in order to predict emissions of the potent greenhouse gas, nitrous oxide (N2O), as well as the other nitrogenous compounds in the pathway. The model predicts increasing N2O emissions as the copper level is lowered, in agreement with experimental observations in chemostats.

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      Analysis of periplasmic sensor domains from Anaeromyxobacter dehalogenans 2CP-C: Structure of one sensor domain from a histidine kinase and another from a chemotaxis protein (pages 766–777)

      P. Raj Pokkuluri, Jeff Dwulit-Smith, Norma E. Duke, Rosemarie Wilton, Jamey C. Mack, Jessica Bearden, Ella Rakowski, Gyorgy Babnigg, Hendrik Szurmant, Andrzej Joachimiak and Marianne Schiffer

      Version of Record online: 30 JUL 2013 | DOI: 10.1002/mbo3.112

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      The Anaeromyxobacter dehalogenans CP-C genome codes for a large number of sensor histidine kinases (HK) and methyl accepting chemotaxis proteins (MCP); among these 23 HK and 11 MCP proteins have a sensor domain in the periplasm. These proteins most likely contribute to adaptation to the organism's surroundings. We predicted their three-dimensional folds and determined the structures of two of the periplasmic sensor domains by X-ray diffraction.

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      The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway (pages 778–794)

      Sandra Cruz, Sofía Muñoz, Elvira Manjón, Patricia García and Yolanda Sanchez

      Version of Record online: 30 JUL 2013 | DOI: 10.1002/mbo3.113

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      We studied SpWsc1p and SpMtl2p, both are proteins with structural features indicative of plasma membrane-associated cell wall sensors. We found that Mtl2p and Wsc1p act by turning-on the Rho1p GTPase. Our results suggest that cell wall assembly in fission yeast is being regulated by two different networks involving Rho1p. One involves signalling from Mtl2p through Rho1p to Pck1p, while the second one involves signalling from Wsc1p and Rgf2p through Rho1p to activate glucan synthase.

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      A simple and novel method for retrieval of Pasteurellaceae from swab samples collected in the field (pages 795–797)

      Mie J. Hansen, Mads F. Bertelsen, Rune Dietz, Christian Sonne and Anders M. Bojesen

      Version of Record online: 30 JUL 2013 | DOI: 10.1002/mbo3.114

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      The article describe a simple, novel method for preserving swab samples until bacterial isolation can be completed in the laboratory.

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      A mutation in the promoter region of zipA, a component of the divisome, suppresses the shape defect of RodZ-deficient cells (pages 798–810)

      Daisuke Shiomi and Hironori Niki

      Version of Record online: 6 AUG 2013 | DOI: 10.1002/mbo3.116

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      We studied the mechanism by which a mutation in the promoter of zipA, which encodes a component of the cell division machinery, restores rod shape in RodZ-deficient E. coli cells. ZipA was slightly increased in the suppressor cells and led to a delay in cell division. Round-shaped rodZ mutants retained cell bipolarity, suggesting that a delay in the completion of septation may provide extra time to elongate the cell laterally.

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      Diverse antimicrobial interactions of halophilic archaea and bacteria extend over geographical distances and cross the domain barrier (pages 811–825)

      Nina S. Atanasova, Maija K. Pietilä and Hanna M. Oksanen

      Version of Record online: 8 AUG 2013 | DOI: 10.1002/mbo3.115

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      Close to 150 halocin production-sensitivity interactions were detected between 90 halophilic archaeal and bacterial strains from numerous geographically distant hypersaline environments. Close to 80% of the interactions were observed between strains from different genera and even domain indicating that different hypersaline environments have similar microorganisms and dynamic antimicrobial interactions.

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      Nitrogen regulation of protein–protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea (pages 826–840)

      Laia Pedro-Roig, Christian Lange, María José Bonete, Jörg Soppa and Julie Maupin-Furlow

      Version of Record online: 28 AUG 2013 | DOI: 10.1002/mbo3.120

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      Here, we report that amtB-glnK operons are highly cotranscribed during conditions of dissimilatory nitrate reduction in Haloferax mediterranei. Furthermore, the GlnK PII regulatory proteins associate with AmtB-type transporters in the membrane of this archaeon after ammonia shock presumably to block ammonia uptake.

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      Fitness and proteome changes accompanying the development of erythromycin resistance in a population of Escherichia coli grown in continuous culture (pages 841–852)

      Denisa Petráčková, Jiří Janeček, Silvia Bezoušková, Ladislava Kalachová, Zuzana Techniková, Karolína Buriánková, Petr Halada, Kateřina Haladová and Jaroslav Weiser

      Version of Record online: 28 AUG 2013 | DOI: 10.1002/mbo3.121

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      Continual cultivation system was used in proteomic analysis of adaptive changes of Escherichia coli culture when grown in the presence of sublethal concentration of erythromycin. In a population growing for more than 100 generations in the presence of antibiotic, more fit and more erythromycin-resistant bacterial clones predominated.

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      Immunity protein release from a cell-bound nuclease colicin complex requires global conformational rearrangement (pages 853–861)

      Mireille Vankemmelbeke, Nicholas G. Housden, Richard James, Colin Kleanthous and Christopher N. Penfold

      Version of Record online: 28 AUG 2013 | DOI: 10.1002/mbo3.122

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      We report here that engineering rigidity in the structured part of the colicin translocation domain through disulfide bond formation prevents immunity protein release from the colicin complex. Reduction of the disulfide bond by the addition of DTT leads to immunity protein release and resumption of activity. We suggest that a global conformational rearrangement along the entire colicin molecule and requiring a cellular energy input is essential for immunity release.

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      Exploration of Deinococcus-Thermus molecular diversity by novel group-specific PCR primers (pages 862–872)

      Nicolas Theodorakopoulos, Dipankar Bachar, Richard Christen, Karine Alain and Virginie Chapon

      Version of Record online: 29 AUG 2013 | DOI: 10.1002/mbo3.119

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      Specific primers targeting the Deinococcus-Thermus phylum were designed and validated in vitro. They were used to explore the molecular diversity of these extremophylic bacteria in desert sand and hot springs from Tunisia.

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      Increased 1-aminocyclopropane-1-carboxylate deaminase activity enhances Agrobacterium tumefaciens-mediated gene delivery into plant cells (pages 873–880)

      Tatsuhiko Someya, Satoko Nonaka, Kouji Nakamura and Hiroshi Ezura

      Version of Record online: 2 SEP 2013 | DOI: 10.1002/mbo3.123

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      Agrobacterium-mediated transformation is a useful tool for the genetic modification of plants, although its efficiency is low for several plant species. The newly developed Agrobacterium tumefaciens with improved ACC deaminase activity showed improved T-DNA delivery into S. lycopersicum and E. ravennae and thus may be a powerful tool for Agrobacterium-mediated genetic engineering of plants.

    15. You have full text access to this OnlineOpen article
      Ecomorphological variability of Arthrospira fusiformis (Cyanoprokaryota) in African soda lakes (pages 881–891)

      Mary Nakabungo Kaggwa, Alfred Burian, Steve Omondi Oduor and Michael Schagerl

      Version of Record online: 2 SEP 2013 | DOI: 10.1002/mbo3.125

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      The filamentous spirally-coiled cyanoprocaryote Arthrospira fusiformis is found in extremely high densities in tropical soda lakes acting as driving force of the food-web. We studied pronounced temporal morphological changes of Arthrospira in Kenyan soda lakes and identified underlying key factors. Percentage of large, widely-coiled filaments representing high vitality was promoted by elevated levels of SRP, wind speed, temperature and conductivity and the opposite for small filaments.

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