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MicrobiologyOpen

Cover image for Vol. 3 Issue 2

April 2014

Volume 3, Issue 2

Pages i–iii, 157–270

  1. Issue Information

    1. Top of page
    2. Issue Information
    3. Original Research
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      Issue Information (pages i–iii)

      Article first published online: 9 APR 2014 | DOI: 10.1002/mbo3.130

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  2. Original Research

    1. Top of page
    2. Issue Information
    3. Original Research
    1. You have full text access to this OnlineOpen article
      Members of the uncultured bacterial candidate division WWE1 are implicated in anaerobic digestion of cellulose (pages 157–167)

      Rim Driss Limam, Rakia Chouari, Laurent Mazéas, Ting-Di Wu, Tianlun Li, Julien Grossin-Debattista, Jean-Luc Guerquin-Kern, Mouldi Saidi, Ahmed Landoulsi, Abdelghani Sghir and Théodore Bouchez

      Article first published online: 5 FEB 2014 | DOI: 10.1002/mbo3.144

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      Using a stable isotope probing methodology combined with secondary ion mass spectrometry-in situ hybridization (SIMSISH), insights were gained about the in situ ecophysiology of uncultivated members of the candidate division Waste Water of Evry 1 (WWE1). NanoSIMS observations using a specific iodized probe revealed that these microorganisms were actively assimilating 13C originating from labeled cellulose during anaerobic digestion.

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      The impairment of methylmenaquinol:fumarate reductase affects hydrogen peroxide susceptibility and accumulation in Campylobacter jejuni (pages 168–181)

      Issmat I. Kassem, Mahesh Khatri, Yasser M. Sanad, Melinda Wolboldt, Yehia M. Saif, Jonathan W. Olson and Gireesh Rajashekara

      Article first published online: 7 FEB 2014 | DOI: 10.1002/mbo3.158

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      We addressed the potential role of an Fe-S containing periplasmic respiratory protein (Mfr) in the response of Campylobacter jejuni to hydrogen peroxide. We found that the impairment of Mfr affects hydrogen peroxide susceptibility and accumulation in C. jejuni. Our study investigated a previously unidentified role for Mfr in hydrogen peroxide susceptibility and accumulation in C. jejuni. Therefore, our study highlights the functional range of respiratory enzymes and their multifaceted role in facilitating the survival of C. jejuni, an important and resilient foodborne pathogen.

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      Growth promotion of the opportunistic human pathogen, Staphylococcus lugdunensis, by heme, hemoglobin, and coculture with Staphylococcus aureus (pages 182–195)

      Jeremy R. Brozyna, Jessica R. Sheldon and David E. Heinrichs

      Article first published online: 7 FEB 2014 | DOI: 10.1002/mbo3.162

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      We demonstrate that S. lugdunensis, an opportunistic human pathogen, grows poorly in iron-restricted media lacking heme or hemoglobin. We demonstrate that this phenotype is due to the combination of having a functional iron-regulated surface determinant system, for acquisition of heme, and nonfunctional or missing genes for synthesis of siderophores. The growth of S. lugdunensis can be significantly enhanced if the species is provided with functional S. aureus genes for SA synthesis or with exogenous supplied siderophores. Moreover, S. lugdunensis growth is also significantly enhanced when grown in coculture with staphyloferrin-producing S. aureus, in a transporter-dependent manner.

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      Fpk1/2 kinases regulate cellular sphingoid long-chain base abundance and alter cellular resistance to LCB elevation or depletion (pages 196–212)

      Yukari Yamane-Sando, Etsuko Shimobayashi, Mitsugu Shimobayashi, Yasunori Kozutsumi, Shogo Oka and Hiromu Takematsu

      Article first published online: 10 FEB 2014 | DOI: 10.1002/mbo3.160

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      We explored new connection between protein kinase and cellular sphingolipids. More specifically, we screened yeast kinome mutants with the acute sphingolipids depletion to identify sphingolipid-related kinase, which we analyzed in detail. (Kinome tree was modified from Hunter T and Plowman GD. Trends Biochem Sci 22, 1997).

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      Cerulenin inhibits unsaturated fatty acids synthesis in Bacillus subtilis by modifying the input signal of DesK thermosensor (pages 213–224)

      Lucía Porrini, Larisa E. Cybulski, Silvia G. Altabe, María C. Mansilla and Diego de Mendoza

      Article first published online: 14 FEB 2014 | DOI: 10.1002/mbo3.154

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      We report that inhibition of fatty acid synthesis in Bacillus subtilis by the antibiotic cerulenin results in increased levels of short fatty acids that lead to inhibition of the transmembrane-input control of DesK thermosensor.

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      Bacteroides isolated from four mammalian hosts lack host-specific 16S rRNA gene phylogeny and carbon and nitrogen utilization patterns (pages 225–238)

      Todd Atherly and Cherie J. Ziemer

      Article first published online: 17 FEB 2014 | DOI: 10.1002/mbo3.159

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      Isolates from the Bacteroides thetaiotaomicron-ovatus-xylanisolvens clade were found in fecal enrichments from mammals of differing digestive physiology, both ruminant and monogastric. These Bacteroides isolates were able to utilize a wide range of the carbon substrates but very few of the nitrogen substrates examined. The rep-PCR analysis indicated that the isolates' genomes had been altered by the host environment to some degree but genomic differences carbohydrate utilization genes. This is the first report to suggest conservation of specific bacterial species with the same functionality across mammalian hosts for this Bacteroidetes clade.

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      Isolation of viable but nonculturable Vibrio cholerae O1 from environmental water samples in Kolkata, India, in a culturable state (pages 239–246)

      Mitsutoshi Senoh, Jayeeta Ghosh-Banerjee, Tamaki Mizuno, Sumio Shinoda, Shin-ichi Miyoshi, Takashi Hamabata, G. Balakrish Nair and Yoshifumi Takeda

      Article first published online: 18 FEB 2014 | DOI: 10.1002/mbo3.164

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      In this study, it was demonstrated that a factor converting viable but nonculturable (VBNC) bacteria into culturable state (FCVC) existed in cell extracts of cultured eukaryotic cells. VBNC Vibrio cholerae O1 from environmental water samples was isolated in culturable state by using thiosulfate citrate bile salts sucrose (TCBS) plates containing FCVC (F-TCBS plates).

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      The glucosaminidase domain of Atl – the major Staphylococcus aureus autolysin – has DNA-binding activity (pages 247–256)

      Inês R. Grilo, Ana Madalena Ludovice, Alexander Tomasz, Hermínia de Lencastre and Rita G. Sobral

      Article first published online: 3 MAR 2014 | DOI: 10.1002/mbo3.165

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      In this communication, we describe the capacity of Atl, the major Staphylococcus aureus autolytic enzyme, to bind DNA. Both the Atl protein and the endo-β-N-acetylglucosaminidase (GL) domain were able to bind DNA of nonspecific sequence. The implications of this unexpected observation for the physiology of Staphylococcus aureus remain to be explored.

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      In silico analysis of AHJD-like viruses, Staphylococcus aureus phages S24-1 and S13′, and study of phage S24-1 adsorption (pages 257–270)

      Jumpei Uchiyama, Iyo Takemura-Uchiyama, Shin-ichiro Kato, Miho Sato, Takako Ujihara, Hidehito Matsui, Hideaki Hanaki, Masanori Daibata and Shigenobu Matsuzaki

      Article first published online: 4 MAR 2014 | DOI: 10.1002/mbo3.166

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      Staphylococcal phage S24-1 belongs to family Podoviridae genus AHJD-like viruses. ORF16 of phage S24-1 was a receptor-binding protein. The ligand molecules to the ORF16 was shown as wall teichoic acids of Staphylococcus aureus.

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