A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli
Article first published online: 8 OCT 2012
© 2012 The Authors. MicrobiologyOpen published by Blackwell Publishing Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 1, Issue 4, pages 381–394, December 2012
How to Cite
MicrobiologyOpen 2012; 1(4): 381–394
- Issue published online: 12 DEC 2012
- Article first published online: 8 OCT 2012
- Manuscript Accepted: 11 SEP 2012
- Manuscript Revised: 9 SEP 2012
- Manuscript Received: 14 AUG 2012
- MEXT (Ministry of Education, Culture, Sports, Science and Technology of Japan). Grant Number: S0801037
- Biofilm formation;
- csgD promoter;
- genomic SELEX;
- regulation network;
- transcription factor
The FixJ/LuxR family transcription factor CsgD is a master regulator of biofilm formation in Escherichia coli. Previously, we identified more than 10 transcription factors that participate in regulation of the csgD promoter. After genomic SELEX screening of regulation targets, an uncharacterized TetR-type transcription factor YbjK was found to be involved in regulation of the csgD promoter. In addition, a number of stress-response genes were found to be under the direct control of YbjK. Taken together, we propose to rename it to RcdA (regulator of csgD). One unique feature of RcdA is its mode of DNA binding. Gel shift, DNase-I footprinting, and atomic force microscopic (AFM) analyses indicated that RcdA is a DNA-binding protein with a high level of cooperativity, with which it covers the entire surface of probe DNA through protein–protein interaction and moreover it induces the formation of aggregates of DNA–RcdA complexes.