Single-cell analysis of cell viability after a biocide treatment unveils an absence of positive correlation between two commonly used viability markers
Version of Record online: 26 DEC 2012
© 2012 The Authors. MicrobiologyOpen published by Blackwell Publishing Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 1, pages 123–129, February 2013
How to Cite
MicrobiologyOpen 2013; 2(1): 123–129
- Issue online: 13 FEB 2013
- Version of Record online: 26 DEC 2012
- Manuscript Accepted: 19 NOV 2012
- Manuscript Revised: 13 NOV 2012
- Manuscript Received: 17 AUG 2012
|mbo362-sup-0001-FigS1.tif||image/tif||650K||Figure S1. Cell elongation of DVC treated and non-treated cells. (A) Sequence of images showing several rounds of cell elongation as a function of time (min) in presence or in absence of ciprofloxacin (4 mg/L). Scale bar = 1 μm. (B) Quantification of the cell length as a function of time (min). The green line gives the cell length of the DVC treated cells. The red line gives the cumulated cell length of the non-treated cells. The dashed red lines give the cell length of the two daughter cells.|
|mbo362-sup-0002-FigS2.tif||image/tif||4373K||Figure S2. Effect of illumination on cell viability using two consecutive sets of CV6 and PBS injections on the same immobilized cells. (A) Layout of the system and injection schedule used for two consecutive sets of CV6 injection. (B) The relationship between the accumulation ratio observed during the first injection of CV6 (T10 min) and the second injection of CV6 (T30 min), when cells were exposed 200 msec (left panel) or 20 msec (right panel). (C, D) Quantification of the cell fluorescence intensity (n = 30) as a function of time (min) when cells were exposed 200 msec (C) or 20 msec (D). The dark line gives the average value of the population observed.|
|mbo362-sup-0003-FigS3.tif||image/tif||3229K||Figure S3. CV6 extrusion profiles were significantly affected by HOCl. (A, B) Quantification of the cell fluorescence intensity (n = 30) as a function of time (min) before HOCl treatment (A) and after HOCl treatment (B). The dark line gives the average value of the population observed.|
|mbo362-sup-0004-MovieS1.avi||video/avi||92K||Movie S1. Cell elongation of DVC treated cells. Sequence of images showing several rounds of cell elongation as a function of time in presence of ciprofloxacin (4 mg/L). Images were taken every 2 min.|
|mbo362-sup-0005-MovieS2.avi||video/avi||3670K||Movie S2. Single-cell analysis of cell activity using ChemchromeV6 (CV6) procedure. Sequence of images showing several rounds of cells stained by CV6 as a function of time. Phase images are on the left panel and fluorescent signal are on the right panel. Images were taken every 2 min.|
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