Identification of quorum sensing-controlled genes in Burkholderia ambifaria
Article first published online: 5 FEB 2013
© 2013 The Authors. MicrobiologyOpen published by Blackwell Publishing Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 2, pages 226–242, April 2013
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How to Cite
Chapalain, A., Vial, L., Laprade, N., Dekimpe, V., Perreault, J. and Déziel, E. (2013), Identification of quorum sensing-controlled genes in Burkholderia ambifaria. MicrobiologyOpen, 2: 226–242. doi: 10.1002/mbo3.67
- Issue published online: 8 APR 2013
- Article first published online: 5 FEB 2013
- Manuscript Accepted: 26 DEC 2012
- Manuscript Revised: 18 DEC 2012
- Manuscript Received: 22 OCT 2012
- Canadian Institutes of Health Research (CIAR)
- Fondation Armand-Frappier
- NSERC summer scholarship
- Fonds de la recherche en santé du Québec (FRSQ)
- Canada Research Chair in Sociomicrobiology
- Antifungal molecules;
- Burkholderia ;
- quorum sensing;
- random mutagenesis;
- secondary metabolites
The Burkholderia cepacia complex (Bcc) comprises strains with a virulence potential toward immunocompromised patients as well as plant growth–promoting rhizobacteria (PGPR). Owing to the link between quorum sensing (QS) and virulence, most studies among Bcc species have been directed toward QS of pathogenic bacteria. We have investigated the QS of B. ambifaria, a PGPR only infrequently recovered from patients. The cepI gene, responsible for the synthesis of the main signaling molecule N-octanoylhomoserine lactone (C8-HSL), was inactivated. Phenotypes of the B. ambifaria cepI mutant we observed, such as increased production of siderophores and decreased proteolytic and antifungal activities, are in agreement with those of other Bcc cepI mutants. The cepI mutant was then used as background strain for a whole-genome transposon-insertion mutagenesis strategy, allowing the identification of 20 QS-controlled genes, corresponding to 17 loci. The main functions identified are linked to antifungal and antimicrobial properties, as we have identified QS-controlled genes implicated in the production of pyrrolnitrin, burkholdines (occidiofungin-like molecules), and enacyloxins. This study provides insights in the QS-regulated functions of a PGPR, which could lead to beneficial potential biotechnological applications.