Identification of quorum sensing-controlled genes in Burkholderia ambifaria
Article first published online: 5 FEB 2013
© 2013 The Authors. MicrobiologyOpen published by Blackwell Publishing Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 2, pages 226–242, April 2013
How to Cite
Chapalain, A., Vial, L., Laprade, N., Dekimpe, V., Perreault, J. and Déziel, E. (2013), Identification of quorum sensing-controlled genes in Burkholderia ambifaria. MicrobiologyOpen, 2: 226–242. doi: 10.1002/mbo3.67
- Issue published online: 8 APR 2013
- Article first published online: 5 FEB 2013
- Manuscript Accepted: 26 DEC 2012
- Manuscript Revised: 18 DEC 2012
- Manuscript Received: 22 OCT 2012
- Canadian Institutes of Health Research (CIAR)
- Fondation Armand-Frappier
- NSERC summer scholarship
- Fonds de la recherche en santé du Québec (FRSQ)
- Canada Research Chair in Sociomicrobiology
Data S1. Supplemental experimental procedures.
Table S1. Primers used in this study.
Table S2. Conditions used in qRT-PCR experiments
Figure S1. Predicted cep-box sequences in Burkholderia species. The detailed method used to determine the putative cep-boxes is described in the explanatory text. All the potential cep-boxes found in the AMMD genome (not only those identified in the screening) are also presented.
Figure S2. Relative expression of candidate quorum sensing-regulated genes by mRNA quantification. The relative expression of the genes was estimated by quantitative reverse transcription PCR (qRT-PCR) experiments on HSJ1 WT and its cepI mutant. The ndh gene was used as reference. The results are expressed as relative quantification of gene expression (log10 scale) in the cepI mutant compared to the WT, normalized to 1. A fold change of two (bottom scale) was chosen as significant threshold. The results are expressed in means ± SD for triplicate assays.
Figure S3. Phenotypic confirmation of transposon mutants for antifungal activities. The antifungal activities of HSJ1 WT, its cepI mutant and three transposon mutants implicated in biosynthesis of pyrrolnitrin, enacyloxins and occidiofungins, were tested against Candida albicans, Pythium ultimum, and Rhizoctonia solani.
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