Roles of the Gac-Rsm pathway in the regulation of phenazine biosynthesis in Pseudomonas chlororaphis 30-84
Version of Record online: 21 APR 2013
© 2013 The Authors. Microbiology Open published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 3, pages 505–524, June 2013
How to Cite
MicrobiologyOpen 2013; 2(3): 505–524
- Issue online: 7 JUN 2013
- Version of Record online: 21 APR 2013
- Manuscript Accepted: 14 MAR 2013
- Manuscript Revised: 13 MAR 2013
- Manuscript Received: 30 JAN 2013
- USDA NIFA NRI. Grant Number: 2008-35319-21879
- NSF. Grant Number: IOS-1035157
|mbo390-sup-0001-FigS1.pdf||application/PDF||101K||Figure S1. (A) Migration phenotypes of WT, gac mutants and 30-84ZN at 48 h after incubation. (B) Comparison of the movement distance of WT, gac mutants and 30-84ZN. Cells were inoculated on the surface of a motility plate containing 0.25% agar as described previously (Wang 2011). Plates were incubated at 28°C for 48 h, during which the displacement diameter to the outermost edge of the movement area was measured. The experiment was performed at least three times in triplicate. (C) Expression levels of fliA, fliC, and fliM in WT and a gacA mutant. RPKM values representing levels of gene expression were calculated from RNA-seq analysis and log transformed. Data points represent means of three replicates ± standard deviations. (D) Verification of RNA-seq data with qPCR. Bacterial strains were grown overnight in LB broth and reinoculated in 5 mL AB medium + 2% casamino acid. Relative expression of fliA, fliC, and fliM genes, normalized to the expression of rpoD gene, was determined by qPCR after ~18 h growth (OD600 at 1.2).|
Table S1. Bacterial strains and plasmids used in this study.
Table S2. Oligonucleotides used for gene cloning and qPCR.
Table S3. Differentially expressed genes in the gacA mutant compared with the WT strain.
Table S4. Mean transcript abundance and ratio of abundances (∆gacA/WT) of type VI secretion system (T6SS) genes in the gacA mutant compared with the WT.
Table S5. Mean transcript abundance and ratio of abundances (∆gacA/WT) of polyhydroxyalkanoate (PHA) biosynthetic genes in the gacA mutant compared with the WT.
Table S6. Mean transcript abundance and ratio of abundances (∆gacA/WT) of regulatory genes in the gacA mutant compared with the WT.
Table S7. Mean transcript abundance and ratio of abundances (∆gacA/WT) of genes involved in protein metabolism in the gacA mutant compared with the WT.
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