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mbo390-sup-0001-FigS1.pdfapplication/PDF101KFigure S1. (A) Migration phenotypes of WT, gac mutants and 30-84ZN at 48 h after incubation. (B) Comparison of the movement distance of WT, gac mutants and 30-84ZN. Cells were inoculated on the surface of a motility plate containing 0.25% agar as described previously (Wang 2011). Plates were incubated at 28°C for 48 h, during which the displacement diameter to the outermost edge of the movement area was measured. The experiment was performed at least three times in triplicate. (C) Expression levels of fliA, fliC, and fliM in WT and a gacA mutant. RPKM values representing levels of gene expression were calculated from RNA-seq analysis and log transformed. Data points represent means of three replicates ± standard deviations. (D) Verification of RNA-seq data with qPCR. Bacterial strains were grown overnight in LB broth and reinoculated in 5 mL AB medium + 2% casamino acid. Relative expression of fliA, fliC, and fliM genes, normalized to the expression of rpoD gene, was determined by qPCR after ~18 h growth (OD600 at 1.2).
mbo390-sup-0002-TableS1-S2_S4-S7.docxWord document41K

Table S1. Bacterial strains and plasmids used in this study.

Table S2. Oligonucleotides used for gene cloning and qPCR.

Table S3. Differentially expressed genes in the gacA mutant compared with the WT strain.

Table S4. Mean transcript abundance and ratio of abundances (gacA/WT) of type VI secretion system (T6SS) genes in the gacA mutant compared with the WT.

Table S5. Mean transcript abundance and ratio of abundances (gacA/WT) of polyhydroxyalkanoate (PHA) biosynthetic genes in the gacA mutant compared with the WT.

Table S6. Mean transcript abundance and ratio of abundances (gacA/WT) of regulatory genes in the gacA mutant compared with the WT.

Table S7. Mean transcript abundance and ratio of abundances (gacA/WT) of genes involved in protein metabolism in the gacA mutant compared with the WT.

mbo390-sup-0003-TableS3.xlsxapplication/msexcel148K 

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