Antibacterial activity of a lectin-like Burkholderia cenocepacia protein
Version of Record online: 5 JUN 2013
© 2013 The Authors. Microbiology Open published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 2, Issue 4, pages 566–575, August 2013
How to Cite
MicrobiologyOpen 2013; 2(4): 566–575
- Issue online: 12 AUG 2013
- Version of Record online: 5 JUN 2013
- Manuscript Accepted: 6 MAY 2013
- Manuscript Revised: 29 APR 2013
- Manuscript Received: 22 MAR 2013
- FWO-Vlaanderen. Grant Number: G.0393.09N
- Belgian Science Policy Office
Table S1. List of strains and plasmids used in this study.
Table S2. List of bacterial growth media tested to trigger LlpA production in B. cenocepacia AU1054. Media were solidified with 1.5% agar. Overlay assays with LlpA-sensitive bacteria after chloroform killing of B. cenocepacia AU1054 were done in LB soft agar (0.5% agar). Media were also tested in coculture, in the latter case cell lawns of indicator bacteria were made in soft agar, after which AU1054 was spotted on top. In parallel, the different growth media were also tested when B. cenocepacia AU1054 was supplemented with mitomycin (1 μg/mL final concentration) prior to spotting. Plates were scored after overnight incubation at 37°C. Generated halos were not pronase sensitive, and not thought to be bacteriocin derived.
Table S3. List of primers used in this study.
Figure S1. Multiple amino acid sequence alignment used to construct the phylogenetic tree of LlpA homologues (Fig. 1). The protein codes and accession numbers are specified in Figure 1. Sequence conservation is visualized by differential shading. The position of the sequences corresponding to QxDxNxVxY-like motifs of MMBL proteins, representing potential carbohydrate-binding pockets, and the separate domains (N- and C-domain with MMBL fold; β-hairpin extension) present in P. putida BW11M1 LlpA (PDB 3M7H) are indicated.
Figure S2. Glycan array profile of LlpA (Bcen_1091 from B. cenocepacia AU1054) as measured by fluorescence intensity. A complete list of tested carbohydrates (array version PA v5) is available from the Consortium of Functional Glycomics (CFG, www.functionalglycomics.org).
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