Get access

Changes in gene expressions elicited by physiological concentrations of genistein on human endometrial cancer cells

Authors

  • N. Konstantakopoulos,

    1. Gynaecological Cancer Research Centre, Royal Women's Hospital, Carlton, Victoria, Australia
    2. Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Victoria, Australia
    Search for more papers by this author
  • K.G. Montgomery,

    1. VBCRC Cancer Genetics Laboratory, Centre for Cancer Genomics and Predictive Medicine, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
    Search for more papers by this author
  • N. Chamberlain,

    1. VBCRC Cancer Genetics Laboratory, Centre for Cancer Genomics and Predictive Medicine, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
    Search for more papers by this author
  • M.A. Quinn,

    1. Gynaecological Cancer Research Centre, Royal Women's Hospital, Carlton, Victoria, Australia
    2. Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Victoria, Australia
    Search for more papers by this author
  • M.S. Baker,

    1. Division of Environmental & Life Sciences Research, Macquarie University, Sydney, Australia
    Search for more papers by this author
  • G.E. Rice,

    1. Gynaecological Cancer Research Centre, Royal Women's Hospital, Carlton, Victoria, Australia
    2. Translational Proteomics, Baker Medical Research Institute, Melbourne, Victoria, Australia
    Search for more papers by this author
  • H.M. Georgiou,

    1. Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Victoria, Australia
    2. Mercy Perinatal Research Centre, Mercy Hospital for Women, Heidelberg, Victoria, Australia
    Search for more papers by this author
  • I.G. Campbell

    Corresponding author
    1. VBCRC Cancer Genetics Laboratory, Centre for Cancer Genomics and Predictive Medicine, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
    • Ian Campbell, VBCRC Cancer Genetics Laboratory, Centre for Cancer Genomics and Predictive Medicine, Peter MacCallum Cancer Institute, St. Andrews Place, East Melbourne 3002, Victoria, Australia.
    Search for more papers by this author

Abstract

The incidence of hormone-related diseases such as prostatic, breast, ovarian, and endometrial cancer is lower in Asian populations compared to Western countries. High consumption of soybean products that are rich in phytoestrogens, predominantly genistein, is postulated to be responsible for the lower incidence of hormone-related disease, although the mechanism through which this effect might be mediated is unclear. In this study, microarray analysis was used to identify the changes in gene expression elicited by treatment of the human endometrial cancer cell line, Ishikawa, with genistein at both physiologically achievable and supraphysiological concentrations. Genistein treatment at 5 µM concentration induced multiple changes in gene expression including some implicated in oncogenesis. In contrast, treatment with a supraphysiological concentration of genistein predominantly activated stress response genes and showed very limited overlap with the genes regulated at lower concentrations. Of the genes regulated by genistein, 9.3% were also regulated by 17β-estradiol suggesting that genistein exerts its response via the estrogen pathway. These results indicate that at physiological concentrations, genistein is able to elicit pleiotropic effects on a variety of pathways believed to be involved in tumorigenesis. Supraphysiological concentrations of genistein, such as those used in many previous studies, elicit changes in gene expression that are unlikely to occur in vivo. © 2006 Wiley-Liss, Inc.

Get access to the full text of this article

Ancillary