Cross reactivity between many anti-human antibodies for their hamster homologs provide the tools to study the signal transduction pathway activated by asbestos and SV40 in the malignant mesothelioma model
Article first published online: 30 APR 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 45, Issue 7, pages 537–542, July 2006
How to Cite
Kroczynska, B. and Carbone, M. (2006), Cross reactivity between many anti-human antibodies for their hamster homologs provide the tools to study the signal transduction pathway activated by asbestos and SV40 in the malignant mesothelioma model. Mol. Carcinog., 45: 537–542. doi: 10.1002/mc.20200
- Issue published online: 11 JUN 2006
- Article first published online: 30 APR 2006
- Manuscript Accepted: 9 JAN 2006
- Manuscript Revised: 5 JAN 2006
- Manuscript Received: 6 DEC 2005
- mesothelial cells;
The aim of this study was to test the possibility of using human antibodies to study the pathogenic mechanism of SV40 and asbestos in a hamster mesothelioma model. The cellular lysates from human and hamster primary mesothelial cells were tested by Western blot analysis. All of the antibodies we tested (HGF, Notch, VEGF, Sp1, p53, PP2A, p-ERK1, p-c-jun, Fra1, Fra2, MMP1, MMP9, NFκB p65, IκB, GAPDH) cross-reacted with their hamster counterparts. These data indicate that hamster mesothelioma model and more in general hamster experimental model, can be used for functional studies because many mouse, rabbit, and goat monoclonal antibodies prepared against human antigens cross-react with their hamster counterparts. © 2006 Wiley-Liss, Inc.