Research Article

Transcriptomic alterations in human prostate cancer cell LNCaP tumor xenograft modulated by dietary phenethyl isothiocyanate

  1. Robert W. Li1,
  2. CongJun Li1,
  3. Thomas T.Y. Wang2,*

Article first published online: 5 FEB 2012

DOI: 10.1002/mc.21873

Additional Information

How to Cite

Li, R. W., Li, C. and Wang, T. T.Y. (2012), Transcriptomic alterations in human prostate cancer cell LNCaP tumor xenograft modulated by dietary phenethyl isothiocyanate. Mol. Carcinog.. doi: 10.1002/mc.21873

Author Information

  1. 1

    U.S. Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Bovine Functional Genomics Laboratory, Beltsville, Maryland

  2. 2

    Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, Beltsville, Maryland

*USDA, ARS, Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, 10300 Baltimore Avenue, Building 307C, Room 132, Beltsville, MD 20705.

Publication History

  1. Article first published online: 5 FEB 2012
  2. Manuscript Accepted: 27 DEC 2011
  3. Manuscript Revised: 15 DEC 2011
  4. Manuscript Received: 28 SEP 2011

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Keywords:

  • RNAseq;
  • prostate cancer;
  • prevention;
  • phenethyl isothiocyate;
  • xenograft

Abstract

Temporal growth of tumor xenografts in mice on a control diet was compared to mice supplemented daily with 3 µmol/g of the cancer preventive compound phenethyl isothiocyanate. Phenethyl isothiocyanate decreased the rate of tumor growth. The effects of phenethyl isothiocyanate on tumor growth were examined by RNAseq to elucidate molecular changes that may contribute to tumor growth suppression. Bio-informatic analysis of differentially expressed genes identified changes in inflammation and extracellular matrix pathways that were modulated by treatment with phenethyl isothiocyanate. Specific gene expression changes in these pathways included up-regulation of insulin-like growth factor binding protein 3, fibronectin, thyroxine degradation enzyme, and down regulation of integrin beta 6. In addition, feeding phenethyl isothiocyanate induced alternative splicing of gene variants. This study represents the first use of RNAseq to analyze tumors from animals consuming dietary phenethyl isothiocyanate and to identify potential molecular signatures that may explain the cancer protective effect of this compound. © 2012 Wiley Periodicals, Inc.

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