LKB1 mutations and their correlation with LKB1 and Rheb expression in bladder cancer
Article first published online: 27 MAR 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 52, Issue 8, pages 660–665, August 2013
How to Cite
Tigli, H., Seven, D., Tunc, M., Sanli, O., Basaran, S., Ulutin, T. and Buyru, N. (2013), LKB1 mutations and their correlation with LKB1 and Rheb expression in bladder cancer. Mol. Carcinog., 52: 660–665. doi: 10.1002/mc.21902
- Issue published online: 12 JUL 2013
- Article first published online: 27 MAR 2012
- Manuscript Accepted: 21 FEB 2012
- Manuscript Received: 7 OCT 2011
- bladder cancer;
- mTOR pathway;
Although there are extensive studies on the genetics of bladder cancer, several questions remain unanswered. One of the pathways which are altered in bladder cancer is the mTOR signaling pathway. In the present study, we analyzed the expression of Rheb gene and genetic alterations in the LKB1 gene which are the key components of mTOR pathway. Nine exons of the LKB1 gene were analyzed by direct sequencing in 51 bladder cancer patients. To investigate the expression of Rheb and LKB1, real-time quantitative RT-PCR was performed in bladder tumor and normal bladder tissue samples. We did not observed a statistically significant difference in Rheb or LKB1 expression between the tumor and normal tissue samples. We detected a novel missense mutation creating stop codon in a high percent of the tumor samples. Five different single nucleotide substitutions were also observed in the introns. Our results indicate that LKB1 gene may play a role in the progression of bladder cancer. © 2012 Wiley Periodicals, Inc.