Interleukin-17 (IL-17) has been shown to play an important role in the pathogenesis of inflammation and autoimmune disorders, and to be elevated in several types of cancer. The present study analyzed polymorphisms in IL-17 gene and their impact on the pathogenesis of bladder cancer. The TaqMan® SNP Genotyping Assay was used to genotype the SNP rs2275913 of IL-17A and SNP rs763780 of IL-17F in 301 bladder cancer patients and 446 ethnicity-matched healthy controls. The frequencies of AA genotype and A allele of rs2275913, as well as TT genotype and T allele of rs763780 in the bladder cancer patients were significantly higher than that of controls, indicating that both of these two SNPs were associated with bladder cancer (P = 0.003, OR = 1.37, 95% CI = 1.12–1.69 for allele A of rs2275913, and P = 0.018, OR = 1.46, 95% CI = 1.07–2.00 for allele T of rs763780, respectively). Results of stratified analysis revealed that rs2275913 was associated with male, non-smokers, and invasion of bladder cancer, while rs763780 was associated with invasion of bladder cancer. Our results suggested that the SNP rs2275913 of IL-17A and SNP rs763780 of IL-17F were associated with the development, as well as gender, smoking status and tumor stage of bladder cancer. © 2012 Wiley Periodicals, Inc.