Transcriptomic analysis by RNA-seq reveals AP-1 pathway as key regulator that green tea may rely on to inhibit lung tumorigenesis

Authors

  • Jing Pan,

    1. Department of Surgery, The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, Missouri
    2. Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin
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  • Qi Zhang,

    1. Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin
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  • Donghai Xiong,

    1. Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin
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  • Peter Vedell,

    1. Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin
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  • Ying Yan,

    1. Department of Surgery, The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, Missouri
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  • Hui Jiang,

    1. Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin
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  • Peng Cui,

    1. Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin
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  • Feng Ding,

    1. Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin
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  • Jay W. Tichelaar,

    1. Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin
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  • Yian Wang,

    1. Department of Surgery, The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, Missouri
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  • Ronald A. Lubet,

    1. Chemoprevention Branch, National Cancer Institute, Bethesda, Maryland
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  • Ming You

    Corresponding author
    1. Department of Surgery, The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, Missouri
    2. Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin
    • Medical College of Wisconsin Cancer Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin. 8701 Watertown Plank Rd, Milwaukee, WI 53226.

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Abstract

Green tea is a promising chemopreventive agent for lung cancer. Multiple signaling events have been reported, however, the relative importance of these mechanisms in mediating the chemopreventive function of green tea is unclear. In the present study, to examine the involvement of AP-1 in green tea polyphenols induced tumor inhibition, human NSCLC cell line H1299 and mouse SPON 10 cells were identified as AP-1 dependent, as these two lines exhibit high constitutive AP-1 activity, and when TAM67 expression was induced with doxycycline, cell growth was inhibited and correlated with suppressed AP-1 activity. RNA-seq was used to determine the global transcriptional effects of AP-1 inhibition and also uncover the possible involvement of AP-1 in tea polyphenols induced chemoprevention. TAM67 mediated changes in gene expression were identified, and within down-regulated genes, AP-1 was identified as a key transcription regulator. RNA-seq analysis revealed that Polyphenon E-treated cells shared 293 commonly down-regulated genes within TAM67 expressing H1299 cells, and by analysis of limited Chip-seq data, over 10% of the down-regulated genes contain a direct AP-1 binding site, indicating that Polyphenon E elicits chemopreventive activity by regulating AP-1 target genes. Conditional TAM67 expressing transgenic mice and NSCLC cell lines were used to further confirm that the chemopreventive activity of green tea is AP-1 dependent. Polyphenon E lost its chempreventive function both in vitro and in vivo when AP-1 was inhibited, indicating that AP-1 inhibition is a major pathway through which green tea exhibits chemopreventive effects. © 2013 Wiley Periodicals, Inc.

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