Dae Ho Ahn and HyungChul Rah contributed equally to this work.
Association of the miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G polymorphisms with gastric cancer risk and survival in the korean population
Article first published online: 21 SEP 2012
© 2012 Wiley Periodicals, Inc.
Special Issue: Genetic Susceptibility to Cancer in Diverse Populations
Volume 52, Issue S1, pages 39–51, November 2013
How to Cite
Ahn, D. H., Rah, H., Choi, Y.-K., Jeon, Y. J., Min, K. T., Kwack, K., Hong, S. P., Hwang, S. G. and Kim, N. K. (2013), Association of the miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G polymorphisms with gastric cancer risk and survival in the korean population. Mol. Carcinog., 52: 39–51. doi: 10.1002/mc.21962
Disclosure: all authors have nothing to disclose.
- Issue published online: 24 OCT 2013
- Article first published online: 21 SEP 2012
- Manuscript Accepted: 23 AUG 2012
- Manuscript Revised: 6 AUG 2012
- Manuscript Received: 23 NOV 2011
- National Research Foundation of Korea
- gastric cancer;
- single-nucleotide polymorphisms;
- genetic susceptibility;
- survival rate
We investigated whether four common microRNA polymorphisms (miR-146aC>G [rs2910164], miR-149T>C [rs2292832], miR-196a2T>C [rs11614913], and miR-499A>G [rs3746444]) are associated with the susceptibility and prognosis of gastric cancer in the Korean population. The four microRNA single-nucleotide polymorphisms (SNPs) were identified in a case–control study (461 patients; 447 controls) by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis in the Korean population. When patients were stratified into diffuse and intestinal-type gastric cancer groups, subjects with the miR-499AG and AG + GG genotypes had reduced adjusted odds ratios (AORs) for diffuse-type gastric cancer (AOR = 0.54 with 95% confidence interval [CI] = 0.31–0.97; AOR = 0.57 with 95% CI = 0.33–0.97). In the stratified analyses for gastric cancer risk, the miR-146aGG and CG + GG genotypes were associated with increased risk of gastric cancers among the non-smokers, whereas the miR-149TC and TC + CC genotypes showed lower risk of gastric cancer in males. The miR-196a2CC genotype was associated with elevated gastric cancer risk among females. For gastric cancer prognosis, intestinal-type gastric cancer patients with miR-146aCG + GG genotypes had significantly higher survival rates (log-rank P = 0.030) than patients with the CC genotype, and patients with the miR-499AA genotype had significantly increased survival rates compared to patients with the AG + GG genotypes (log-rank P = 0.013). When miR-146aCG + GG and miR-499AA genotypes were combined, the survival rate of intestinal-type gastric cancer patients was elevated (log-rank P < 0.001). No association was found between gastric or diffuse-type cancer prognosis and other miRNAs. Our data demonstrate that specific miRNA SNPs are associated with gastric cancer susceptibility (miR-499A>G) and prognosis (miR-146aC>G and miR-499A>G) in the Korean population depending on gastric cancer type. © 2012 Wiley Periodicals, Inc.