Decreased expression of kallikrein-related peptidase 13: Possible contribution to metastasis of human oral cancer
Article first published online: 31 JAN 2013
© 2013 Wiley Periodicals, Inc.
Volume 53, Issue 7, pages 557–565, July 2014
How to Cite
Ishige, S., Kasamatsu, A., Ogoshi, K., Saito, Y., Usukura, K., Yokoe, H., Kouzu, Y., Koike, H., Sakamoto, Y., Ogawara, K., Shiiba, M., Tanzawa, H. and Uzawa, K. (2014), Decreased expression of kallikrein-related peptidase 13: Possible contribution to metastasis of human oral cancer. Mol. Carcinog., 53: 557–565. doi: 10.1002/mc.22007
- Issue published online: 17 JUN 2014
- Article first published online: 31 JAN 2013
- Manuscript Accepted: 4 JAN 2013
- Manuscript Revised: 26 DEC 2012
- Manuscript Received: 17 AUG 2012
- kallikrein-related peptidase 13;
- oral squamous cell carcinoma;
- adhesion molecules
The human kallikrein-related peptidase family is comprised of 15 serine protease genes on chromosome 19q13.4 . Our previous microarray analyses showed that the gene kallikrein-related peptidase 13 (KLK13) was down-regulated in oral squamous cell carcinoma (OSCC) cell lines. We evaluated the expression status of KLK13 in primary OSCCs and performed functional molecular experiments in OSCC cell lines. In 102 primary tumors studied, KLK13 expression significantly (P < 0.05) decreased compared with matched normal counterparts. Interestingly, KLK13-negative cases correlated significantly (P < 0.05) with regional lymph node metastasis. In vitro, cells overexpressing KLK13 (oeKLK13) had decreased invasiveness and motility and up-regulation of adhesion molecules (E-cadherin, α-catenin, β-catenin, junction plakoglobin, plakophilin4, desmocollin2, desmoglein3, and desmoplakin) compared with control cells. A rescue experiment that transfected oeKLK13 cells with siRNA against KLK13 restored invasiveness and migration activities with down-regulated adhesion molecules. Based on our results, we concluded that KLK13 may play an important role in regulating cellular migration and invasiveness, making the loss of KLK13 a potential biomarker for early detection of lymph node metastasis in OSCCs. © 2013 Wiley Periodicals, Inc.