The molecular chaperone cosmc enhances malignant behaviors of colon cancer cells via activation of Akt and ERK

Authors

  • John Huang,

    1. Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
    2. Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
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  • Mei-Ieng Che,

    1. Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei, Taiwan
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  • Neng-Yu Lin,

    1. Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei, Taiwan
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  • Ji-Shiang Hung,

    1. Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
    2. Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
    3. Department of Medical Research, National Taiwan University Hospital, Taipei, Taiwan
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  • Yu-Ting Huang,

    1. Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei, Taiwan
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  • Wei-Chou Lin,

    1. Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan
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  • Hsiu-Chin Huang,

    1. Animal Technology Institute Taiwan, Miaoli, Taiwan
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  • Po-Huang Lee,

    1. Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
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  • Jin-Tung Liang,

    Corresponding author
    1. Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
    • Division of Colorectal Surgery, Department of Surgery, National Taiwan University Hospital and College of Medicine, No. 7, Chung-Shan South Road, Taipei 100, Taiwan.

      Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, No. 1, Sec. 1 Jen-Ai Road, Taipei 100, Taiwan.

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  • Min-Chuan Huang

    Corresponding author
    1. Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei, Taiwan
    2. Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan
    • Division of Colorectal Surgery, Department of Surgery, National Taiwan University Hospital and College of Medicine, No. 7, Chung-Shan South Road, Taipei 100, Taiwan.

      Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, No. 1, Sec. 1 Jen-Ai Road, Taipei 100, Taiwan.

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  • The authors have declared that no conflict of interest exists.
  • Authors' contributions: M.I.C., N.Y.L., Y.T.H., and H.C.H. designed and performed experiments. J.H., J.S.H., W.C.L., and P.H.L. participated in collecting the clinical data. J.T.L. and M.C.H. participated in designing and coordinating the study, and in writing the manuscript. All authors read and approved the final manuscript.

Abstract

Expression of T antigen (Galbeta1, 3GalNAc) is associated with enhanced metastatic potential and poor prognosis in colorectal cancer. Cosmc is a molecular chaperone required for the formation of an active T-synthase, which catalyzes the synthesis of T antigen. However, the expression and role of Cosmc in colorectal cancer are still unclear. Here, real-time PCR showed that overexpression of Cosmc mRNA in colorectal tumors compared with paired non-tumorous tissues was associated with increased American Joint Committee on Cancer (AJCC) tumor stage. Forced expression of Cosmc in HCT116 cells significantly increased T antigen expression and enhanced cell growth, migration, and invasion, which was associated with increased phosphorylation of focal adhesion kinase (FAK), ERK, and Akt. These Cosmc-enhanced malignant phenotypes were significantly suppressed by specific inhibitor of MEK or PI3K. We also found that Cosmc overexpression increased tumor growth and decreased survival of tumor-bearing SCID mice. Conversely, knockdown of Cosmc with siRNA in SW480 cells decreased malignant behaviors and the signaling pathways, which were substantially reversed by constitutively active Akt or MEK. Taken together, these results suggest that Cosmc promotes malignant phenotypes of colon cancer cells mainly via activation of MEK/ERK and PI3K/Akt signaling pathways, and that Cosmc may serve as a potential target for colorectal cancer treatment. © 2013 Wiley Periodicals, Inc.

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