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Anti-lymphangiogenesis effects of a specific anti-interleukin 7 receptor antibody in lung cancer model in vivo

Authors

  • Ming Jian,

    1. No. 202 Hospital of People Liberation Army of China, Shenyang, P.R., China
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  • Zhang Qingfu,

    1. Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, P.R., China
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  • Jiang Yanduo,

    1. No. 202 Hospital of People Liberation Army of China, Shenyang, P.R., China
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  • Jiang Guocheng,

    1. No. 202 Hospital of People Liberation Army of China, Shenyang, P.R., China
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  • Qiu Xueshan

    Corresponding author
    1. Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, P.R., China
    • Correspondence to: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, No. 92 North Second Road, Heping District, 110001 Shenyang, P.R. China.

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  • Ming Jian and Zhang Qingfu contributed equally to this work as co-first authors.
  • Conflict of interest: The authors declare that they have no competing interests.

Abstract

Interleukin 7 (IL-7) is known to promote lymphangiogenesis. To study the relationship between IL-7 and the lymphangiogenesis in lung cancer cells xenograft tumors, we investigated how IL-7 regulates lymphangiogenesis by Quantitative real-time reverse transcriptase–polymerase chain reaction, Western blot, co-immunoprecipitation, chromatin immunoprecipitation, and immunohistochemistry methods. We found that, in lung cancer cells xenograft tumors IL-7/IL-7 receptor (IL-7R) increase the expression of VEGF-D and lymphangiogenesis, induce c-Fos and c-Jun heterodimer formation, and enhance c-Fos/c-Jun DNA binding activity to regulate VEGF-D. Taken together, our results provided evidence that IL-7/IL-7R induce VEGF-D upregulation and promote lymphangiogenesis via c-Fos/c-Jun pathway in lung cancer. © 2013 Wiley Periodicals, Inc.

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