Original Paper
Separation, detection, and identification of inositol triphosphate and phytic acid derivatives by supercritical fluid chromatography and SFC–mass spectrometry
Article first published online: 30 MAR 2005
DOI: 10.1002/mcs.1220010405
Copyright © 1989 Aster Publishing Corporation
1520-667X/asset/cover.gif?v=1&s=838ec380d02860ce73da5173cdca2a348cbc0575 "Journal of Microcolumn Separations")
Additional Information
How to Cite
Chester, T. L., Pinkston, J. D., Innis, D. P. and Bowling, D. J. (1989), Separation, detection, and identification of inositol triphosphate and phytic acid derivatives by supercritical fluid chromatography and SFC–mass spectrometry. J. Micro. Sep., 1: 182–189. doi: 10.1002/mcs.1220010405
Publication History
- Issue published online: 30 MAR 2005
- Article first published online: 30 MAR 2005
- Abstract
- References
- Cited By
Keywords:
- supercrirical fluid chromatography;
- mass spectrometry;
- capillary column;
- inositol;
- phosphate;
- phytic acid;
- silyl derivatives
Abstract
Capillary supercritical fluid chromatography with flame ionization detection appears promising for the separation and detection of silylated inositol phosphates. Inositol triphosphate and phytic acid, following ion exchange to their fully protonated forms, were silylated in one step with the addition of 9 and 12 trimethylsilyl groups, respectively. The resulting derivatives are well behaved in open-tubular chromatography columns with methyl-silicone stationary phase when CO2 is used as the mobile phase. Both flow-splitting and directinjection methods were evaluated. The identities of the derivatives were confirmed by supercritical fluid chromatography-mass spectrometry using chemical ionization with both ammonia and isobutane, and using electron ionization—CO2 charge exchange.