Quantification of acetogenins in Annona muricata linked to atypical parkinsonism in guadeloupe

Authors

  • Pierre Champy PharmD, PhD,

    1. Laboratory of Pharmacognosy, UMR 8076 Centre National de la Recherche Scientifique (CNRS) BioCIS, Faculté de Pharmacie Paris XI, Châtenay-Malabry, France
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  • Alice Melot PharmD,

    1. Laboratory of Pharmacognosy, UMR 8076 Centre National de la Recherche Scientifique (CNRS) BioCIS, Faculté de Pharmacie Paris XI, Châtenay-Malabry, France
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  • Vincent Guérineau Eng,

    1. Laboratory of Mass Spectrometry, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique (CNRS) Gif-sur-Yvette, France
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  • Christophe Gleye PharmD, PhD,

    1. Laboratory of Pharmacognosy, UMR 8076 Centre National de la Recherche Scientifique (CNRS) BioCIS, Faculté de Pharmacie Paris XI, Châtenay-Malabry, France
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  • Djibril Fall PharmD, PhD,

    1. Laboratory of Pharmacognosy, UMR 8076 Centre National de la Recherche Scientifique (CNRS) BioCIS, Faculté de Pharmacie Paris XI, Châtenay-Malabry, France
    2. Laboratory of Therapeutic Chemistry, Faculté de Pharmacie, Université Cheikh Anta Diop, Dakar, Senegal
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  • Gunter U. Höglinger MD, PhD,

    1. Institut National de la Santé et de la Recherche Médicale (INSERM) U679, Experimental Neurology and Therapeutics, Paris, France
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  • Merle Ruberg PhD,

    Corresponding author
    1. Institut National de la Santé et de la Recherche Médicale (INSERM) U679, Experimental Neurology and Therapeutics, Paris, France
    • INSERM U679 (formerly U289), Hôpital de la Salpêtrière, 47 Bd de l'Hôpital, 75013 Paris, France
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  • Annie Lannuzel MD, PhD,

    1. Institut National de la Santé et de la Recherche Médicale (INSERM) U679, Experimental Neurology and Therapeutics, Paris, France
    2. Department of Neurology, Centre Hospitalier Universitaire (CHU) Antilles-Guyane, Pointe-à-Pitre, Guadeloupe, France
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  • Olivier Laprévote PharmD, PhD,

    1. Laboratory of Mass Spectrometry, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique (CNRS) Gif-sur-Yvette, France
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  • Alain Laurens PharmD, PhD,

    1. Laboratory of Pharmacognosy, UMR 8076 Centre National de la Recherche Scientifique (CNRS) BioCIS, Faculté de Pharmacie Paris XI, Châtenay-Malabry, France
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  • Reynald Hocquemiller PharmD, PhD

    1. Laboratory of Pharmacognosy, UMR 8076 Centre National de la Recherche Scientifique (CNRS) BioCIS, Faculté de Pharmacie Paris XI, Châtenay-Malabry, France
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Abstract

Atypical parkinsonism in Guadeloupe has been associated with the consumption of fruit and infusions or decoctions prepared from leaves of Annona muricata L. (Annonaceae), which contains annonaceous acetogenins, lipophilic inhibitors of complex I of the mitochondrial respiratory chain. We have determined the concentrations of annonacin, the major acetogenin in A. muricata, in extracts of fruit and leaves by matrix-assisted laser desorption-ionization mass spectrometry. An average fruit is estimated to contain about 15 mg of annonacin, a can of commercial nectar 36 mg, and a cup of infusion or decoction 140 μg. As an indication of its potential toxicity, an adult who consumes one fruit or can of nectar a day is estimated to ingest over 1 year the amount of annonacin that induced brain lesions in rats receiving purified annonacin by intravenous infusion. © 2005 Movement Disorder Society

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