Characterization of peripheral hematopoietic stem cells and monocytes in Parkinson's disease
Funding agencies: This study was supported by the Gemeinnützige Hertie-Stiftung and the University of Tübingen.
Relevant conflicts of interest/financial disclosures: Nothing to report.
Full financial disclosures and author roles may be found in the online version of this article.
Author Roles: Natalja Funk and Saskia Biskup designed the study, Natalja Funk and Peter Wieghofer performed all experiments, Sabrina Grimm helped with the flow-cytometric experiments, Richard Schaefer helped in establishing and analyzing the CFC assay, Hans-Jörg Bühring and Thomas Gasser helped with critical discussions, and Natalja Funk, Peter Wieghofer, and Saskia Biskup wrote the manuscript. All authors read and approved the final manuscript.
Correspondence to: Saskia Biskup, Hertie Institute for Clinical Brain Research and German Center for Neurodegenerative Diseases, Otfried-Mueller Str. 27, 72076 Tuebingen, Germany. E-mail: email@example.com
Emerging evidence has highlighted the pivotal role of the immune system in neurodegenerative diseases. This study investigated the impact of progressive neurodegeneration on the differentiation and development of hematopoietic stem cells in the peripheral blood of Parkinson's patients. Methods: A colony-forming cell assay was established to study hematopoietic stem cells from venous blood of Parkinson's patients, and flow cytometry was used to analyze the expression of chemokine receptors on monocytes.
We demonstrate that there is strong upregulation in the percentage of monocyte precursors in the peripheral blood of Parkinson's patients and asymptomatic high-risk individuals. We identify the receptor CCR2 as undergoing strong upregulation on the surface of classical monocytes in Parkinson's patients.
The association between blood cell development and progressive cell death in the brain of Parkinson's patients should be further investigated as a potential dynamic biomarker and indicator of disease progression. © 2013 Movement Disorder Society