Natalja Funk and Peter Wieghofer contributed equally to this work.
Characterization of peripheral hematopoietic stem cells and monocytes in Parkinson's disease
Article first published online: 7 FEB 2013
Copyright © 2012 Movement Disorders Society
Volume 28, Issue 3, pages 392–395, March 2013
How to Cite
Funk, N., Wieghofer, P., Grimm, S., Schaefer, R., Bühring, H.-J., Gasser, T. and Biskup, S. (2013), Characterization of peripheral hematopoietic stem cells and monocytes in Parkinson's disease. Mov. Disord., 28: 392–395. doi: 10.1002/mds.25300
Funding agencies: This study was supported by the Gemeinnützige Hertie-Stiftung and the University of Tübingen.
Relevant conflicts of interest/financial disclosures: Nothing to report.
Full financial disclosures and author roles may be found in the online version of this article.
Author Roles: Natalja Funk and Saskia Biskup designed the study, Natalja Funk and Peter Wieghofer performed all experiments, Sabrina Grimm helped with the flow-cytometric experiments, Richard Schaefer helped in establishing and analyzing the CFC assay, Hans-Jörg Bühring and Thomas Gasser helped with critical discussions, and Natalja Funk, Peter Wieghofer, and Saskia Biskup wrote the manuscript. All authors read and approved the final manuscript.
- Issue published online: 21 MAR 2013
- Article first published online: 7 FEB 2013
- Manuscript Accepted: 31 OCT 2012
- Manuscript Revised: 20 OCT 2012
- Manuscript Received: 29 APR 2012
- The Gemeinnützige Hertie-Stiftung and the University of Tübingen
- Parkinson's disease;
- innate immunity
Emerging evidence has highlighted the pivotal role of the immune system in neurodegenerative diseases. This study investigated the impact of progressive neurodegeneration on the differentiation and development of hematopoietic stem cells in the peripheral blood of Parkinson's patients. Methods: A colony-forming cell assay was established to study hematopoietic stem cells from venous blood of Parkinson's patients, and flow cytometry was used to analyze the expression of chemokine receptors on monocytes.
We demonstrate that there is strong upregulation in the percentage of monocyte precursors in the peripheral blood of Parkinson's patients and asymptomatic high-risk individuals. We identify the receptor CCR2 as undergoing strong upregulation on the surface of classical monocytes in Parkinson's patients.
The association between blood cell development and progressive cell death in the brain of Parkinson's patients should be further investigated as a potential dynamic biomarker and indicator of disease progression. © 2013 Movement Disorder Society