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mds25493-sup-0001-suppfig1.tif8662KSUPPLEMENTARY FIG 1. Low-magnification photomicrographs showing the cytoarchitectural distribution of the dopaminergic cell groups identified using tyrosine hydroxylase immunohistochemistry at the 3 rostrocaudal levels of the human midbrain. These levels were identified in each case and adjacent section double-labeled and assessed for fluorescence intensity analysis as detailed in the Patients and Methods section. The most caudal level (C) was at the decussation of the superior cerebellar peduncle (xscp). An intermediate level (B) where the greatest number of exiting third-nerve fibers occurred was assessed. The most consistent rostral level (A) was just below the opening of the aqueduct into the third ventricle at the greatest cross-sectional extent of the red nucleus. At this rostral level there were many ascending tyrosine hydroxylase immunopositive axons in the medial aspects of the midbrain. Scale in C is equivalent for A and B (cp, cerebral peduncle; R, red nucleus; SNC, substantia nigra pars compacta; VTA, ventral tegmental area).

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