Bionanotechnology based on silica nanoparticles

Authors

  • Weihong Tan,

    Corresponding author
    1. Center for Research at the Bio/nano Interface, Department of Chemistry and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611
    2. State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Biological Technology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
    • Center for Research at the Bio/nano Interface, Department of Chemistry and McKnight Brain Institute, University of Florida, Gainesville, FL 32611.
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  • Kemin Wang,

    Corresponding author
    1. Center for Research at the Bio/nano Interface, Department of Chemistry and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611
    2. State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Biological Technology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
    • State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Biological Technology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
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  • Xiaoxiao He,

    1. State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Biological Technology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
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  • Xiaojun Julia Zhao,

    1. Center for Research at the Bio/nano Interface, Department of Chemistry and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611
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  • Timothy Drake,

    1. Center for Research at the Bio/nano Interface, Department of Chemistry and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611
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  • Lin Wang,

    1. Center for Research at the Bio/nano Interface, Department of Chemistry and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611
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  • Rahul P. Bagwe

    1. Center for Research at the Bio/nano Interface, Department of Chemistry and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611
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Abstract

We have developed uniform core/shell nanoparticles, consisting of a silica layer coating and pigments or magnetite core, using a water-in-oil microemulsion method. The nanoparticles are highly luminescent and photostable with the size ranging from 5 nm to 400 nm. Bioconjugation of these silica nanoparticles adds unique biofunctions with various molecules such as enzymes, antibodies, and DNA molecules. Significant advantages have been shown in using bioconjugated nanoparticles for biosensing and bioimaging, such as cell staining, DNA detection and separation, rapid single bacterium detection, and biotechnological application in DNA protection. © 2004 Wiley Periodicals, Inc. Med Res Rev, 24, No. 5, 621–638, 2004

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